18-4 - Chapter Iii Paper and Liquid Chromatography band...

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Unformatted text preview: Chapter Iii Paper and Liquid Chromatography band width). A better way of expressing the efficiency is lo use the height commie»: in o- theoretic-oi plate (H), where L H:— N and where the column is the porous stationary phase and L is its length. Modern highvperfornnanee chromatography instruments can provide many thousands of plates per meter of column {i.er. H < ill nun]; consequently. the component bands are eauaordinarily nafl'ow. These high efficiencies have been achieved by teclutologieal developments in the manufacture of solid stationary phases and the tubes {columns} in which the phases are contained. In high-pedbmnce iiquia’ ehmmrogmpinv {HPLCL the particles of stationary phase are often spherical. very smali (diameters less than 5 a 10'5 m}. and packed into the tube in a highly uniform manner. One unfortunate aspect of all this cfiiciency is that it has become very difficult to force the mobile phase through the very small particles packed into a tube. MostLrnodern HPLC inshumehts contain very hi 3h pressure pumps to force the mobile phase through the column at reasonable flow rates. 18-4 flfll‘lflflflflflflflflflfl ...
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