Unformatted text preview: Chapter II Pep-er and Liquid Chromatography volunteer mohilephase E 3
volumenf stationary phase I limping. In an actual PC separation the Rf value is deﬁned experimentally by Rf _ distance moved by the cornponent
distance moved by the mobile phase from As you can see. the relative migration distance Rfdepends on the chemical nature of the eonmoneut and mobile phase. In a chromatographic experiment in which a sampie
mixture containing several components is applied to the paper at the start, the Rf value of each component will
depend on the partition ooeiﬁcient for each component in the system enthoyed. The discussion thus far has been restricted to the chromatoyaphy of a sin LIQUID CHROMATOGRAPHY Liquid chromatography [LC] is the name that is now universally used to describe ehrnnntogrephjc
separations meted out in mnall mbcdar columns peeked wi ‘ 13-8 J i ' BEHPIPJPJPJIJDJI'JFJ' i. i. i- i. 1' 1| il l. 1' ii I. 1. 1. 1| II n 1- ...
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- Spring '08