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Chp4_VantHoffdata - 2.5E6 25 1.5E6 29 1.3E6 37 4.5E5 In...

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Van’t Hoff Plot Lecture Problem The following data were obtained for the binding of a ss-DNA oligonucleotide to bacterial recombinase RecA- . See J. Biol. Chem. 276(13), 9613-19, 2001. (partial) Raw Data: Temperature (ºC) K (equilibrium constant) 22
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Unformatted text preview: 2.5E6 25 1.5E6 29 1.3E6 37 4.5E5 In practice, one would run a linear regression analysis via calculator or spreadsheet; here, we will try the two-point fit with two random points, e.g. 25ºC and 37ºC say. Solution to be worked in class....
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