Homework_2 - AEP 470, Fall 2008 Due date: Monday,...

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AEP 470, Fall 2008 Homework problem set # 2 Due date: Monday, October-20-2006 1. (15 points) Which effect, limiting the practical resolution of a light microscope image can be largely removed by image processing, confocal microscopy, two-photon microscopy, or TIRF microscopy? What are the respective advantages and disadvantages of these four different techniques? Why is a detector pinhole needed in the confocal microscope but not in the 2-photon microscope? Do these methods improve the theoretical resolution criteria known as Abbe’s resolution? To study movement of fluorescently labeled vesicles (diameter 40 nm) in axons of hippocampal neurons (axon diameter ~0.2μm). Which type of fluorescence microscopy would you apply: conventional wide field, confocal, two photon, or TIRF? Explain Why. 2. (20 points) In electron microscopy phase contrast is generated by spherical aberration and defocus. In class we derived the phase shift γ between the scattered wave and the unscattered wave as function of scattering angle to be " = # 2 $ ( C S % 4 2 z 2 ) , where C S is spherical aberration constant, θ is the scattering angle and z is the defocus. Maximal phase contrast is achieved when this phase shift is
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This note was uploaded on 03/29/2009 for the course A&EP 470 taught by Professor Lindau during the Fall '08 term at Cornell University (Engineering School).

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Homework_2 - AEP 470, Fall 2008 Due date: Monday,...

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