Lec9_MolecularForces_ML

Lec9_MolecularForces_ML - Molecular Forces Biophysical...

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1 Biophysical Methods Slide 1 Molecular Forces Biophysical Methods Slide 2 Molecular Forces moving trap fixed trap Single vesicle manipulation using an optical tweezer Vesicle.avi
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2 Biophysical Methods Slide 3 Molecular Forces The dependence of dissociation kinetics on applied force F=0 F>0 Biophysical Methods Slide 4 Molecular Forces The dependence of dissociation kinetics on applied force F=0 F>0 We consider the dissociation of a bond where a complex of two binding partners dissociates into its parts with a rate constant k : AB k " # " A + B Usually we need to consider also the reverse reaction of re- binding. AB k " # $ $ A + B However, in the presence of an applied external pulling force we may ignore rebinding because the components A and B will be pulled away from each other.
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3 Biophysical Methods Slide 5 Molecular Forces The dependence of dissociation kinetics on applied force F=0 F>0 8 6 4 2 0 V (arbitrary units) -3 -2 -1 0 1 2 3 Å Biophysical Methods Slide 6 Molecular Forces The dependence of dissociation kinetics on applied force F=0 F>0
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4 Biophysical Methods Slide 7 Molecular Forces The dependence of dissociation kinetics on applied force F=0 F>0 This fraction will be given by the Boltzmann Distribution and the rate will be proprtional to a Boltzmann factor: k " e # E A k B T Biophysical Methods Slide 8 Molecular Forces The dependence of dissociation kinetics on applied force F=0 F>0 Now we apply an external force.
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This note was uploaded on 03/29/2009 for the course A&EP 470 taught by Professor Lindau during the Fall '08 term at Cornell.

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Lec9_MolecularForces_ML - Molecular Forces Biophysical...

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