Syllabus - A&EP 470 BIONB 470 VETMM 470 BME 570 Biophysical...

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A&EP 470 – BIONB 470 – VETMM 470 – BME 570 Biophysical Methods Instructor: Prof. Manfred Lindau An overview of the diversity of modern biophysical experimental techniques used in the study of biological systems at the cellular and molecular level as will be found in articles published in the Biophysical Journal. Topics covered include methods that examine both structure and function of biological systems. Topics include light microscopy, fluorescence microscopy, Fourier optics and image processing, confocal and multiphoton microscopy, Evanescent wave microscopy and Fluorescence correlations spectroscopy, phase contrast, electron microscopy, x-ray diffraction and protein structure determination, multidimensional NMR, spectroscopy, chromophores, calcium measurements, resonance energy transfer, membrane biophysics, electrophysiology, ion channels, action potentials, ligand-gated channels, fluctuation analysis, patch-clamp, molecular biology of ion channels, rapid kinetics, caged compounds, transmitter release, capacitance measurements, amperometry, optical traps, and molecular force measurements, as well as computer modeling. The course format includes a weekend symposium where the students present specific topics such as additional techniques or specific applications, typically based on literature reviews or own research results. The
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