L23 DNA cloning - DNA cloning 1 DNA cloning Making many...

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DNA cloning 1
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DNA cloning Making many copies of a gene Insertion of gene into a vector Making cDNA from RNA Restriction mapping PCR - polymerase chain reaction 2
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Why clone genes? Make many copies It’s easier to manipulate cloned genes: To study genes and/or gene products (protein) To produce large quantities of protein (for medical purposes, for example) To prepare to insert a gene in another organism (animal or plant) 3
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Two ways to make copies of genes* Insert gene into vector, express it in bacteria ( in vivo ) Polymerase Chain Reaction (PCR) ( in vitro ) * or any DNA fragment of interest (promoter, telomere, etc) http://inventorspot.com/Fles/images/e%20coli.img_assist_custom.JPG http://upload.wikimedia.org/wikipedia/commons/8/81/PCR_tubes.png 4
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Cloning DNA in bacteria: a DNA cloning kit Vector (plasmid) Gene of interest DNA ligase restriction enzymes 5
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Vectors DNA with an origin of replication into which another DNA sequence can be introduced example: bacterial plasmids plasmids often have antibiotic resistance genes in order to replicate in host, must contain sequences recognized by the host 6
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This note was uploaded on 03/29/2009 for the course BIO 325 taught by Professor Saxena during the Fall '08 term at University of Texas at Austin.

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L23 DNA cloning - DNA cloning 1 DNA cloning Making many...

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