281lectureAlani - WT 0 4 6 8 12 2.5 5 7 10 24 0 4 6 8 12...

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Why do we need genetic recombination? Genome Integrity Genetic Diversity Segregate away deleterious mutations 5’ 3’ 5’ 3’ 5’ 3’
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Recombination plays an important role in restarting stalled replication forks
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For most eukaryotes, crossover recombination is important for chromosome disjunction at Meiosis I
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For most eukaryotes, crossover recombination is important for chromosome disjunction at Meiosis I Hochwagen and Amon 2006
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A Model For How Crossovers Are Formed In Meiosis
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Keeney et al. Cell 88:375 SPO11, RAD50 How are double strand breaks formed ? 3’ 3’
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A A DSB probe 0 5.1 7.6 KB A recombination hot spot
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Meiotic double strand break sites in yeast Chromosome III Southern Blot Analysis Baudet et al.
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Single-ended Invasion intermediate Formation of the Single-ended Invasion intermediate
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Mazin et al. Mol. Cell 6, 583 Biochemistry of strand invasion
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Single-ended Invasion intermediate Double Holliday Junction Intermediate
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Mom Dad DSB site 1kb Probe X X X X P P P P LEU2 HIS4 N R S his4X LEU2 N R S 33c 33c 0 4 6 8 12 2.5 5 7 10 24
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Unformatted text preview: WT 0 4 6 8 12 2.5 5 7 10 24 0 4 6 8 12 2.5 5 7 10 24 0 4 6 8 12 2.5 5 7 10 24 csm4 Mom Dad DSBs COs Ects 5 hr dHJs SEIs 0 2.5 4 5 6 7 8 10 12 24 Southern Blots 1-dimensional 2-dimensional Keun Kim and Nancy Kleckner SEIs, dHJs Mom Dad COs Ects Oh et al. Mol. Cell 31:324 Holliday Junctions detected by Electron Microscopy Meiosis: one round of DNA replication followed by two rounds of cell division Hochwagen and Amon 2006 Steps in chromosome synapsis Are these steps linked to the recombination steps that lead to crossover formation? Mom Dad short axial cores short axial cores with synaptonemal complex medium axial cores with synaptonemal complex nearly complete synaptonemal complex complete synaptonemal complex complete synaptonemal complex SPO11, RAD50 Do chromosomes form synaptonemal complexes in the absence of genetic recombination? rad50 null, short axial elements that are unpaired rad50S , long axial elements that are unpaired...
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This note was uploaded on 03/30/2009 for the course BIO 2810 taught by Professor Fox/nero during the Fall '07 term at Cornell University (Engineering School).

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281lectureAlani - WT 0 4 6 8 12 2.5 5 7 10 24 0 4 6 8 12...

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