Chem 114A 2009 lec 7 AS GIVEN 012709

Chem 114A 2009 lec 7 AS GIVEN 012709 - Chem 114A...

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Chem 114A Transcription and Translation Lecture 7 January 27, 2009 (Tuesday) 1
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1953 1981 Fiber diffraction of B form DNA. 2 Review
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Structure of DNA. Review
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Axial view of DNA structure shows stacking of bases and hydrogen bonds across the strands. 4 Review
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Principal features of DNA structure. Two helical polynucleotide chains are coiled around a common axis. The chains run in opposite directions. The sugar-phosphate backbones are on the outside. The bases are on the inside. The bases are nearly perpendicular to the helix axis. Adjacent bases are separated by 3.4 A. The helical structure repeats every 34 A. There is a rotation of 36 degrees per turn. The diameter of the helix is 20 A. Since individual AT and GC base pairs have nearly the same size, the structure is independent of sequence. 5 Review
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Replication: Not as simple as it seems. “It has not escaped our notice that the specific pairing we have postulated immediately suggests a possible copying mechanism for the genetic material.” (Watson and Crick) 6
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