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QuizNov3 quiz 2 - addition of this buffer do to the...

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BIO 325L Quiz Week of November 3, 2008 Name: __________________________________ TA: _________________________ 1. Approximately 85% of all genetically modified crops have one or two common sequences. List the two sequences below. Give the one word description for their job/function. 1. Either 35S promoter or cauliflower mosaic virus (CaMV 35S) is fine. If they don’t say 35S promoter, they must say that CaMV 25S is a promoter. 2. NOS terminator or nopaline synthase terminator. 2. In Phase 1 of the Protein Purification lab, you and your partner were told to pin down the contents of the microtube. What does this specific step do? Concentrates the bacteria. 3. During HIC, we added binding buffer to the bacterial lysate, What does the
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Unformatted text preview: addition of this buffer do to the structure of GFP? The end result is that the supernatant containing GFP has the same salt concentration as the equilibrated column. When in a high salt solution, the hydrophobic regions of the proteins are more exposed and are able to interact with and bind the hydrophobic regions of the column. This paragraph was copied from the readings for their homework. They must at least say that the high salt solution causes the hydrophobic regions of the protein to be more exposed. 4. List two of the buffers used in HIC during the protein purification experiment: Any two of these four: Equilibrium Buffer, Binding Buffer, Wash Buffer, Elution Buffer...
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