Unformatted text preview: addition of this buffer do to the structure of GFP? The end result is that the supernatant containing GFP has the same salt concentration as the equilibrated column. When in a high salt solution, the hydrophobic regions of the proteins are more exposed and are able to interact with and bind the hydrophobic regions of the column. This paragraph was copied from the readings for their homework. They must at least say that the high salt solution causes the hydrophobic regions of the protein to be more exposed. 4. List two of the buffers used in HIC during the protein purification experiment: Any two of these four: Equilibrium Buffer, Binding Buffer, Wash Buffer, Elution Buffer...
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- Fall '08
- DNA, hydrophobic regions, elution buffer, 35S promoter, high salt solution