03 MICROSCOPY 2 2009 - Some lessons from last lab 1....

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Unformatted text preview: Some lessons from last lab 1. Importance of illumination and proper adjustment of the condenser 2. How to find objects, especially if they are transparent Fluorescence Microscopy fluorescence imaging has become the mainstay of microscopy -- Nat. Methods, 2005 Objectives: 1. Understand the basics of fluorescence 2. Understand how fluorophores are used in microscopy 3. Understand how a microscope is set up to do fluorescence 4. Understand basics of immunofluorescence Have you used a fluorescence microscope before? A. Yes B. No Fluorescence microscopy uses substances called fluorophores or fluorochromes as stains. These substances have the property that they fluoresce, i.e. they absorb some wavelength of light, become excited, and decay to the ground state by emitting a slightly longer wavelength of light. R O Y G B I V 700 650 600 550 500 450 400 E = h wavelengths in nm http://www.chroma.com/products/cataloghttp://www....
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This note was uploaded on 04/01/2009 for the course BIO 49125 taught by Professor Allen during the Spring '09 term at University of Texas at Austin.

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03 MICROSCOPY 2 2009 - Some lessons from last lab 1....

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