L10_CHEM480_JCCYu

L10_CHEM480_JCCYu - Introduction to Analytical Separations...

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1 Introduction to Analytical Separations ± Extraction: the transfer of a solute from one phase to another. ± To isolate the desired analyte ± To concentrate the desired analyte ± To separate the desired analyte form interferences. 2 Recall! 1. Concentrations of solutes should be expressed as moles per liter. 2. Concentrations of gases should be expressed in bars. 3. Concentrations of pure solids, pure liquids, and solvents are omitted because they are unity. 3 Ex. Equilibrium Constant The equilibrium constant for the reaction H 2 O ± H + + OH ± is called K w (= [H + ][OH ± ]) and has the value 1.0 ² 10 ± 14 at 25°C. Given that K HN3 = 1.8 ² 10 ± 5, for the reaction NH 3 ( aq ) + H 2 O ± NH 4 + + OH ± , find K for the reaction NH 4 + ± NH 3 ( aq ) + H + .
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2 Common Sample Preparation Techniques ± Liquid-liquid extraction ± Conventional liquid lquid extraction ± Micro liquid liquid extraction ± Micro drop liquid liquid extraction ± Membrane supported liquid liquid extraction ± Solid phase extraction ± Conventional solid phase extraction ± Immunoaffinity column extraction ± Molecularly imprinted solid phase extraction ± Micro-disc solid phase extraction ± Micro solid phase extraction ± Stir bar extraction Liquid-liquid Extraction ± Organic solvent that are Immiscible with water: ± Diethyl ether, toluene, hexane (less dense than water) ± Chloroform, dichloromethane,
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L10_CHEM480_JCCYu - Introduction to Analytical Separations...

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