L12_CHEM480_JCCYu - Polarized Light Microscope Nikon...

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1 Polarized Light Microscope Nikon Micrscope U: http://www.microscopyu.com/ http://micro.magnet.fsu.edu/primer/index.html Varieties of Microscopes ± Simple Microscope ± Compound Microscope ± Stereo Microscope ± Biological Microscope ± Polarized Light Microscope ± Comparison Microscope ± Confocal Microscope ± Non-optic Microscope ± Scanning Electron Microscope ± Scanning Probe Microscopes (Atomic Force Microscope, Chemical Force Microscope, Near-field Scanning Optical Microscopy) Diagram of a Microscope
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2 Normal Orthscopic Aperture Conoscopic Diffraction Conjugate Planes in Optical Microscopy ± Two sets of principal conjugate focal planes ± Four field planes (the field or image- forming conjugate set set) ± Four aperture planes (the illumination conjugate set) Conoscopic (Aperture mode) Orthscopic (Normal mode) http://www.microscopyu.com/tutorials/java/conjug ateplanes/index.html Köhler Illumination ± The basic requirements of Köhler illumination: ± A collector lens on the lamp housing is required to focus light emitted from the various points on the lamp filament at the front aperture of the condenser while completely filling the aperture. ± Simultaneously, the condenser must be focused to bring the two sets of conjugate focal planes (when the specimen is also focused) into specific locations along the optical axis of th i the microscope. ± Meeting these conditions will result in a bright, evenly illuminated specimen plane, even with an inherently uneven light source such as a tungsten-halogen lamp filament (the filament will not be in focus in the specimen plane). ± With the specimen and condenser in focus, the focal conjugates will be in the correct position so that resolution and contrast can be optimized by adjusting the field and condenser aperture diaphragms. http://www.microscopyu.com/tutorials/java/kohler/index.html
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3 Conjugate Focal Planes Aperture or Illuminating Conjugate Plane Set Field or Image-Forming Conjugate Plane Set Microscope Exit Pupil: Eye Iris Diaphragm, Ramsden Disc, and Eyepoint Retina of the Eye Camera Image Plane Objective Rear Focal Plane
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This note was uploaded on 04/02/2009 for the course CHM 480 taught by Professor Yu during the Spring '09 term at Sam Houston State University.

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L12_CHEM480_JCCYu - Polarized Light Microscope Nikon...

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