2009 BIOSC 102 CLASS

2009 BIOSC 102 CLASS - Cellular Biomineralization : A new...

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Cellular Biomineralization : A new paradigm for shell formation Dr. Andrew S. Mount, Ph.D. Department of Biological Sciences Clemson University mount@clemson.edu 316 Jordan
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Cellular Biomineralization New paradigm : Cells nucleate and deposit crystals at the biomineralization front Mount et al, 2004 Science 304:299- 300 Current research is focused on understanding the re-modeling process of crystals to produce complex prismatic layers Future research will focus on the interaction between crystal making
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Heart- kidney Percardial axis Hemocoel Outer Mantle Epithelia EPF Shell
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Shell formation can be induced Gulf Coast oyster – 48 hours after notching with a cement saw
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Two layers of oyster shell Outer Prismatic layer Inner Foliated layer Normal Induced
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REF cell Granulocytes
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Refractive (REF) cells are very motile
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Refractive granuloyctes, which circulate in the hemolymph, are crystallogenic DIC SEM SEM-EDS
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A. Backscatter detector – showing crystal B. Secondary detector – showing cell C. Pseudocolor merge– showing crystal inside of cell Crystals are formed inside hemocytes
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to the mineralization front
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This note was uploaded on 04/03/2009 for the course CH 102 taught by Professor Staff during the Spring '08 term at Clemson.

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2009 BIOSC 102 CLASS - Cellular Biomineralization : A new...

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