exIII-2 - GENETICS 310 Practice EXAM III-2 I. Fill in the...

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
GENETICS 310 Practice EXAM III-2 I. Fill in the missing bases in the following 6 base-pair restriction endonuclease cut sites: 1) A C T _ _ _ 2) G _ _ T _ _ _ _ _ T C A _ _ _ _ A _ 2) Shotgun clones can be made using only 2 types of enzymes. What are they? 3) With regard to the preparation of cDNA clones: a) Name two enzymes needed to make double stranded cDNA b) Why is making cDNA easier starting with eukaryotes than prokaryotes? c) What advantage do cDNA clones have over shotgun clones? 4) In regard to plasmid vectors: a) What is the function of the "ori" region? b) What is the value of a gene for ampicillin resistance? c) What advantage comes from placing a multiple cloning site in the beginning of the lacZ gene?
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
II. Two normal parents have a child with cystic fibrosis. Each parent has one normal allele and one defective allele, as revealed by the bands on the Southern blot shown below. They have elected to have a prenatal exam. a) Is the fetus at risk for also having cystic fibrosis?
Background image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 04/29/2008 for the course GENE 310 taught by Professor Magill during the Spring '08 term at Texas A&M.

Page1 / 4

exIII-2 - GENETICS 310 Practice EXAM III-2 I. Fill in the...

This preview shows document pages 1 - 3. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online