Lecture 3-Light and Cells

Lecture 3-Light and Cells - Lecture 3- Light and Cells...

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Lecture 3- Light and Cells Microscopy Fluorescence (stained organism) -Dye molecule accepts a photon of light at one amount of energy, and reflecting it at different energy Transition Electron Microscopy (TEM) -“Sliced” cells stained with electron dense stain and blown with electrons through cells Scanning Electron Microscopy (SEM) -Cells coated on outside with electron dense stain, electrons bounce off them Light Detection in Prokaryotes -Bacteriorhosopsin (a protein) made by ribosomes, manufactured in cytosol -mRNA transcribed from DNA sent to ribosomes telling them to make proteins -Rhodopsin genes are around the edge of the nucleoid because it is a membrane protein (allows for convenient transfer to membrane) 3 Domains ****What is the deal with this slide? Bacteria vs. Archea -Bacteria have no membranous organelles, one RNA polymerase, no histones with DNA, and a circular chromosome. -Archaea have no membranous organelles, several RNA polymerases, histones with DNA,
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Lecture 3-Light and Cells - Lecture 3- Light and Cells...

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