Lecture 002 - Egg Whites & Afros

Lecture 002 - Egg Whites & Afros - Lecture 3 Egg...

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Unformatted text preview: Lecture 3: Egg whites & afros Bio1222 The 'Central Dogma' Bio1222 1 The control of protein abundance Bio1222 Transcriptional / post-transcriptional Bio1222 Gene 2 Northerns and Westerns • Northern - Transcript abundance ST 0 Aox1 rRNA mRNA 1 1.5 2 3 4 8 12 24 h Bio1222 • Western - Protein abundance AOX COXIII Protein Amino acids & peptides Bio1222 3 Protein structure: Primary Bio1222 Protein Structure: Secondary Bio1222 4 Protein Structure: Tertiary Bio1222 Thiol groups - Odours & Afros • Thiol (sulfhydryl) group -S-H Bio1222 Protein-CH2-SH + HS-CH2-Protein Protein-CH2-S-S-CH2-Protein 5 Protein structure: Quaternary • Two or more polypeptides Bio1222 Substrate 1 H e m o g lo b in Substrate 2 Substrate 3 Heme The importance of prosthetic groups • Chlamydomonas - green algae – Wild-type (WT) & mutant (M) Amino acid synthesis Bio1222 NO3 Nitrate reductase (NR) NH4 WT NR M WT NH4 NO3 M 6 Protein denaturation • Loss of native conformation (tertiary structure) Bio1222 Fried eggs & Egg whites Bio1222 Ovalbumin Cream of tartar (potassium bitartrate) Copper bowl 7 Protein folding • A protein’s conformation determines function!! – Human genome project • 20,000-25,000 protein-encoding genes Bio1222 Chaperonins • Many proteins need help to fold correctly Bio1222 • Heat shock proteins (HSPs). 8 ...
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