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Unformatted text preview: RNA sequences for GU followed by AG reveals that-- (1) No GU in this RNA. (2) There is a GU (nucleotides 3 &4) but no AG after the GU. (3) There are several GU in this RNA, but no AG after the first GU. (4) There are several GU in this RNA and one AG near the 3'-end. Therefore, there is a potential intron here. (5) There are multiple GU in this RNA, and two AG downstream from the GU. Therefore, there is a potential intron here. A search for the branch-point sequence (YNCURAY; N= any nucleotide; Y=Pyrimidine; R=Purine) reveals that only RNA #5 has such a sequence (UACUAAC). Therefore, probably only RNA #5 has an intron. In this RNA, only one of the 2 AG is downstream from the branch point sequence. After splicing the intron out, the RNA sequence will be either 5- UAG/UCUCA-3 or 5'-UAGGUUCGCAUUGAC/UCUCA-3' (/ indicates splice point)...
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