EXAMreview1

EXAMreview1 - Study Guide exam 3 B & D sections Labs...

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Labs 12-16 plus “The Spectrophotometer”, “Making Solutions”, and “PAGE” (7-3 to 7- 7) Practice Questions 1. A solution of 0.004 % protein is made. The absorption coefficient at 280 nm in a 1 cm cuvet for a 1% solution is 6.4. What should be the absorbance if the 0.004% solution was made correctly? (show calculation) 2. The molecular weight of the protein is 55,000. What is the molarity of the 0.004% solution? (show calculation) 3. You want to make a restriction digest with 4 tubes containing a total volume of 50 ul each. You decide to make enough master mix for 5 tubes. (a) How much 10X buffer should you add to the master mix? (b) You want 3 μ g of DNA in each tube (5 tubes total). When you put 2 μ l of your DNA stock solution into a cuvet with 1 ml water the A260 is 0.12. How many μ l of the stock solution should you add to the master mix? (show calculations) 4. (a) What is the function of the NaOH in the lysis buffer used to extract plasmid DNA
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This note was uploaded on 04/29/2008 for the course BIBC 103 taught by Professor Coleman during the Winter '08 term at UCSD.

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EXAMreview1 - Study Guide exam 3 B & D sections Labs...

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