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EXAMreview1

# EXAMreview1 - Study Guide exam 3 B D sections Labs 12-16...

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Study Guide exam 3 B & D sections Labs 12-16 plus “The Spectrophotometer”, “Making Solutions”, and “PAGE” (7-3 to 7- 7) Practice Questions 1. A solution of 0.004 % protein is made. The absorption coefficient at 280 nm in a 1 cm cuvet for a 1% solution is 6.4. What should be the absorbance if the 0.004% solution was made correctly? (show calculation) 2. The molecular weight of the protein is 55,000. What is the molarity of the 0.004% solution? (show calculation) 3. You want to make a restriction digest with 4 tubes containing a total volume of 50 ul each. You decide to make enough master mix for 5 tubes. (a) How much 10X buffer should you add to the master mix? (b) You want 3 μ g of DNA in each tube (5 tubes total). When you put 2 μ l of your DNA stock solution into a cuvet with 1 ml water the A260 is 0.12. How many μ l of the stock solution should you add to the master mix? (show calculations) 4. (a) What is the function of the NaOH in the lysis buffer used to extract plasmid DNA from the bacteria?

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EXAMreview1 - Study Guide exam 3 B D sections Labs 12-16...

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