Lecture 2-new09

Lecture 2-new09 - Egg Blastula Tadpole 1 cell 10,000 cells...

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Egg Blastula Tadpole 1 cell 10,000 cells 1,000,000 cells 6 hours 110 hours
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Model systems Amphibians (Xenopus) Zebrafish Drosophila Mouse Chicken Nematode large eggs , easy to obtain and fertilize eggs develop externally fragment culture transparent eggs genetics embryos similar to mammals easier to obtain and observe embryo culture organogenesis cell death Advantages Uses embryology , induction RNA and protein localization morphogenesis cell cycle RNA and protein localization morphogenesis cell determination mammal with relatively short generation time genetics organogenesis segmentation cell determination genetics , gene cloning short generation time genetics transparent embryo small number of cells, invariant lineages axis formation segmentation and ce ll identity signaling pathways organe lle movement cell fate decisions signal transduction ALSO: Sea urchins, ascidians, amphioxus, plants
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Timing and cell numbers in early embryos 25,000 ce lls 75,000 ce lls 100,000 cells 15,000 ce lls Gurdon (1992) Ce ll 68, 185-199
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Stages of development Gilbert Fig. 2.1 Blastula Germ plasm Hatching
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Fertilization Cleavage Divisions
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This note was uploaded on 04/07/2009 for the course BIO 4301 taught by Professor Hillis during the Spring '08 term at Baylor.

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Lecture 2-new09 - Egg Blastula Tadpole 1 cell 10,000 cells...

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