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Lecture1new - Stem cells, cloning, and related issues Stem...

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Unformatted text preview: Stem cells, cloning, and related issues Stem cells self-renew and descendants differentiate to repopulate multiple cell lineages Types of mammalian stem cells Totipotent : potential to generate all the cells that make up an embryo and support its development (p.1380) Pluripotent : potential to give rise to cells derived from all three embryonic germ layers: mesoderm, endoderm, ectoderm Embryonic : derived from the inner cell mass (ICM) of a blastocyst, are pluripotent and self-renewing Adult/somatic : specialized stem cells that form different types of tissues in the body Blastocyst formation and the inner cell mass 1. Inject into another blastocyst - does it contribute to all tissues? 1. Inject into adult mice - cells form teratomas. Are all germ layers (endoderm, mesoderm, and ectoderm) represented? 1. Allow cells to differentiate in vitro - do "embryoid bodies" contain cells from all germ layers? How to show a cell is pluripotent: Cells from the inner cell mass can be cultured in vitro Differentiate into multiple lineages in vivo and in vitro MBC23-68 Embryonic Stem (ES) cells: Self renew and grow indefinitely under proper culture conditions. Isolated from rodents, humans, primates Many potential uses including therapeutic cloning, gene therapy, reproductive cloning, regenerative medicine. Introduction of ES cells by micro-injection into recipient blastocyst MBC 22- 91 Cultured ES cells can be manipulated:- Introduce DNA encoding normal gene into ES cells with mutation- Target a gene for homologous recombination to eliminate its expression- Introduce modified ES cells into recipient blastocyst to generate mice containing an altered gene so ES cells can be used to manipulate the genomes (and phenotypes) of animals. Briggs and King, 1952 Reproductive Cloning vs. Therapeutic Cloning The goal of reproductive cloning is to produce offspring...
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This note was uploaded on 04/07/2009 for the course BIO 4301 taught by Professor Hillis during the Spring '08 term at Baylor.

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Lecture1new - Stem cells, cloning, and related issues Stem...

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