Molecular biology techniques

Molecular biology techniques - Molecular biol Na me...

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Molecular biology techniques Name Molec type used for description Electrophoresis Dna (or rna) Characterizati on Size Run through gel Southern blot Dna Characterizati on Use probe w/ labeled dna Sequencing/genomics Dna Characterizati on Fragments differ in length by 1 base Electrophoresis/pcr Northern blot Rna Characterizati on Probed rna marker Comparison to size/abundance Cdna microarrays Rna Characterizati on Differences in expression of sp. genes b/tw two tiss Abundance of each mrna expressed Western/immune- blotting Protein Characterizati on Antibody binding Separated by size by electrophoresis Size/abundance Name Molec type used for description Transgenics Dna Localization Add a gene Integrate foreign dna In situ hybridization Rna Localization Time/location Uses complementary rna/dna to bind to tissue Immunostaining Protein Localization 1*ab attached, attach 2*ab to
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This note was uploaded on 04/07/2009 for the course ZOO 470 taught by Professor Hardin during the Spring '08 term at Wisconsin.

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Molecular biology techniques - Molecular biol Na me...

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