Bacteriology 303- Exam 2- Spring 05

Bacteriology 303- Exam 2- Spring 05 - ’Y’ Exam 2...

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Unformatted text preview: ’Y’ Exam 2 rPagelofll Bacteriology 303 Second Examination March 15, 2005 The overall reaction for the t‘ennentation of glucose by Lactobacillus acidophilus is given in the pathway below. Numbers refer to the individual enzymatic reactions of the pathway. Glucose -mer3‘1£§lticosa.*25 . 1:3 Memoirs-seas ”-4P- - :Fzruetoaaéiafiiutéfi ‘ s . (145132???EYES-rafteeewee-{Efi.3fii4F-ialwiar’atsy"-1-. , - 7i ' a a . ;t'2;12«P€G5fyeasrate 4 , ~‘ -2 .PhaspheanaipymatefizPyzavate The overall-reaction is: CGHIZOG --------- > 2 OBI-1603 (lactic acid) + 56 kcal (per mole glucose) 1. The cleavage enzyme in this pathway that identifies it as the Embden-Meyerhof pathway is A. phosphoketolase _ . B. fructose 1,6-diphosphate aldolase ' ' . ' ' ' . 7 C. hexose kinase ' E D. KDPG aldolase . IE. lactate dehydrogenase ' 2; The oxidation reaction that is mediated by the coenzyme NAD is reaction number , 3. Substrate-level phosphorylation that leads to the synthesis of ATP occurs at reaction A.l B.4 D.6 ' - _ _ '- E. 10 A. l . . B. 5 ~ _ . ' . C. 6 _ 7 ' ~ . D. 10 ' _ ' , . L E. two of the above ‘ ' 4. Assuming that the bacterium transpOrted the glucose using a binding protein—dependent transport system which utilized ATP for transport, what would be the net gain in ATP per mole of glucose obtained by this fermentation? A. 1 mole B.2moles ' ' . . . _ _ . C.3moles . ' ' . ,-,D.4moles,,,,.,,---..,..,.-,-...72...-”"n- n,-,.......- ._.-n-,.,-,.--.-.,,-,.n,.,,.v._-.,,. -....._,,.- ., , -. .,2. E. none of the above 5; What is the efficiency of this fermentation? (Assume ATP--—-:-——-> ADP + Pi + 8 kcal/mole) http://wwwbactwisc.edu/Bact303/Exam2 _ i _ , 5/6/2005 //. ,Examz . ' . : PageZoflflt A. 10 percent ‘ B. 15 percent. ' ‘ ‘ \C C. 29 percent ' ‘ _ D. 38 percent I ‘ E. 44 percent Questions 6—9 refer tothe following sequences of reactions from bacterial metabolic pathways. pyruvm acid *1 we: S-fihespheglfieefiieefim . ieLkainLesatoxy’figphfipfioglnmnieacid .I: ': ‘tl’i‘ ' ‘ -dismissalddhydeéasphwpfidté 3‘ tiaififfiirasphcgtynafle-ac‘id' ' , _ _ . ‘ .. phosphate- 6. Which of the pathways shown are used by the yeast, Saccharomyces cerevisiae, during fermentation that produces ethanol and CO ‘? ' 2 A.'B,A . . \ , 4 B. C,A,B . c. D,B,A,E ' 907% wt.- EN. D. D B A > > - M04 in - E. E only . ' . - - _ . yff 9 ll mks ' V g 0 l6 ' Which pathways are used by the bacterium, Zymomona, to produce ethanol and C02? http://www.bact.wisc.edu/Bact303/Exam2 - - . ' 5/6/2005 fl ,‘Examl , . , V Page3ofll 8. Which pathways are used by Streptococcus to produce ethanol, lactate and col? i A. B, A B. D, B,A C. D, B,E D. C, B, A E. none of the above 9. Which sequence is common to all fennentative bacteria (all pathways of glycolysis)? . A. A ' B. B ' j ' . ' c. c ' B‘ D. D ' ‘ - i ‘ What end products are distinctive for fennentations carried out by the clostridia ‘2 . acetone B. butanol , . i ' . , 1 C.' butyric acid . , D D. all of the above , ’ ' . ' E. none of the above What bacterial end product is distinctive of the tennentations of non fecal enterics (e g KIebsieIIa) and does not occur in the fecal enteiics (e g. E coll")? A. lactose B. lactic acid C. butanol D. indol E. none of the above 12- Coupling proton motive force to the sy nthesis of ATP is a characteristic of all of the following ty pes of bacterial metabolism except A. lithotrophy B. phototrophy . p ,1 C. fermentation _ . . . C D. aerobic respiration I E. anaerobic respiration 13. During heterotrophic respirations in procaryotes A. the TCA cycle is employed for the complete oxidation of an organic substrate B. ATP is produced by' the process of electron transport phosphorylation _ ‘ ‘ C. ATP (or GTP) is produced by the process of substrate-level phosphorylation E D. oxygen may not be used as a final electron acceptor E. all of the above Questions 14— 17 refer to the following metabolic pathway for the aerobic utilization of lactate by AlcaIigenesfaecalis. Numbers refer to the individual enzy matic reactions of the pathway. http://vvww_.bact.wisc.edu/Bact3 O3/Exam2 . ' 4 . 5/6/2005 ExamZV - , , Page4ofll. \ Lactate: ‘I."~.:.“.' . ,Pymvatg—ma». .. . .fifiefiflé‘cififi' :31' i * ,Ak' WaxCSUQEBZ .10 N. -: Malaie—i—ammma-r Oxaleaeetaté“ , ’[g . a 'Fumarate V laminate: ya; 3 ~ ' . ‘a Is; Seminars Mammyls‘cea w: .. arlphaaewgtulsraze . l4. Substrate—level phosphorylations that lead to the synthesis of ATP (or GTP) occur at reactions A. 2 - .B. 5 . ~ \ C. 2 and 6 . ' ' V\ D. 7 V i » i ‘ E. 8 15. Decarboxylations occur at reactions A. 2 only ' B. 2 and 6 only C. 6 only I ‘ _ D. 5 and 6 only ‘ . V * ‘ A E. 2, 5 and6 _ Which sequence below correctly lists all reactions in the pathway Where NADH2 is produced? ALG. ' BJJflfiJO _ ' _ \fi' CZJfiJO , ' ' , _ Egg Dziaam,- ' g . \ E. none of the above I I 17. Which reaction(s) involve oxidations mediated by flavin adenine dinucleotide? A. l ' B. 2 and 6' eiammo _ i . D8 » ' ' /[> E. none of the above . .’ Questions 18-20 refer to the following metabolic pathway for the utilization of glucose by aerobically-rewiring E. coli and Bacillus subtilis. i ' http://www.bact.wisc.edu/Bact3 03/Exam2 . 5/6/2005 Exam~2 ' t ' I . . . Page 5 of 11 . £441: bat-tinny , .ar «Jami/sis ’1 Glucesemmfi Fyruuate semen-zest . + _____1. . . sacnrazev .9 :2 Malena 2‘ Oxaleaeétafta 2.3.11. amate- , . 2 .‘Esncttt'ate ESE]: mate {WWW—~12 enticing; 131355 <n—.——_—_21 =13. pfiamkatbgjfuéaraia The overall reaction is: CGH 1206 + 6 02—— ------- > 6 CO2 + 6 H20 + 688 kcal/mole glucose In E. coli what 15 the net 3 ield In high energ3 phosphate (= ATP or GTP) per mole of glucose when this pathway is coupled to an electron transport phosphor3 lation s3 stern? (P/O= 2 for E. coli) A. 20 . _ - B. 22 ' ' » < ‘ , C. 26 - /~' D. 36 E. 38 What is the overall efficienc3 of this respiration in E. coli ‘7 (Assume ATP --------- > ADP + Pi + 8 kcal/mole) A. 26% , B. 30% - . - c. 38% (1’7 D; 44% > , ' ‘ \' ' E. cannot be calculated from the data given 29: What 1s the eflicienc3 of this 1espiration in Bacillus '7 (P/O= 3 for Bacillus) ' IA. 18% B. 36% _ V 3 . c. 38% _ ' _ D. 44% ‘ _ E. cannot be calculated from the data given 21. Components of the electron transport systems of both aerobic (respiring) and phototrophic bacteria include A. ferredoxin B. bacteriochlorophyll C. quinones , > . D. cytochromes ' ‘/ E. two of the above 22. Which equation below represents the overall process of bacterial denitrification by Bacillus or Pseudomonas? A. N2 >2NH3 B NH ' >NO2 ...c.No.3 213.113 - .- .. ..... ---- é) .---- -.--. D. No2 >No3 - - E. none of the above http://Www.bact.wisc.edu/Bact303/Exam2 . . . 5/6/2005 Exam2 , , ' , . " Page6of111 \ 23. The biological process of nitrification, as conducted by the bacterium Nitrosomonas, involves A. reduction of N2 B. oxidation of NH3 ‘ ‘ . ‘ i C. feduction of NO3 ' D. reduction ofNO2 ‘ . E E. none of the above Most lithotrophs are also A. autotrophs . »' I I ' I B. phototrophs , W B C. aerobic respirers D. anaerobic respirers E. two of the above 25. LithOtrophic oxidations include all of the following except A. H2 .> 21-120 . B. Fe” . . > Fe+++ c. so > st D. s0 S so 4 ‘ E. none of the above (all are lithotrophic oxidations) 13.st ' >5o 27. The light reactions of photosynthesis in anoxygenic phototrophs involve A. photosystem I only B. photosystem 11 only . . I . ' 9-7ph9tpsystem IrandphotosystemWII , WWW WW ,, ,, "WA/"W gift/COW VD. chlorophyll a - . _ ‘, v E. phy‘cobilins . WON i/ V http://vaw.bact.wisc.edu/Bact3 O3/Exam2 ‘ ' - 5/6/2005 .1 4 high “A.“ B. regeneration of CO2 for autotrophic CO2 fixation C“ 13me . - ' 7. Page7of11 Photoautotrophic growth in the Purple Bacterium Rhodobacter involves A. chlorophyll that absorbs light in the range of 650-750nm B. fixation of CO2 by RUBP carboxylase and the Calvin cycle ‘ / C. use of a substance other than 02 as a final electron acceptor ' Z; D. production of O2 3 i E. two of the above 29. During the light reactions of photosystem II in the cyanobacteria _ A. an electron is ejected and transferred to a pigment in photosystem I _ B. ATP is produced by the mechanisms of electron transport phosphorylation b C. 02 is produced D. all of the above E. none of the above 30. An important role of carotenoids in all photosy nthetic bacteria, besides acting as light-harvesting pigments, involves A shielding chlorophyll trom harmful wavelengths of light B. "quenching" singlet oxy gen, thereby preventing harmful photooxidations in cells C. transferring electrons in the photosynthetic electron transport system , p T; D. providing electrons to photosy stem I for reduction of ferredoxin ' ' ‘ i E. all of the above 3 l. The function of the Calvin Cycle in autotrophs includes A. regeneration of ribulose bisphosphate necessary to diive the cycle C. regeneration of NADPHZaS a source of reductant D. production of intermediates for cell biosynthesis (anabolism) E. two of the above 32. During CO2 fixation involving RUBP carbOxylase and the Calvin cycle, how many 'NADPHZ are required to reduce CO2 to glucose (CGHIZOG)? ' i I _A.1 ' , ‘ - ' . v, 13.2 . _ ._ g (3.4 . _ - . D. 10 E. 12 33. The Calvin cycle is an example of a(n) A. anabolic pathway ' . ' i B. catabolic pathway C. ainphibolic pathway D. all of the above E. none of the above 34. Which reaction below is involved in autotrophic CO2 fixation in cyanobacteria? A. Acetyl CoA + CO2 --------- > Pyruvate _ VB succinyl CoA l- CO2 -_.: ..... > alpha Ketoglutarate D C. alpha Ketoglutarate + CO2 --------- > lsocitrate D. Ribulose bisphosphate + CO2 --------- > (2) 3—P-Glycerate http://wwwbactwisc.edu/Bact3 03/Exam2 _ i ‘ 5/6/2005 ExamZ ' C 7 , . Pag'eS'ofll E. coZ + 2H+— ------- > co 35. Which reaction below is involved in autotrophic CO2 fixation in the Green Bacteria?‘ A. Phosphoenolpyruvate + CO2 --------- > Oxalacetate B. Suecinyl CoA + CO2 --------- > alpha Ketoglutarate I ' g C. alpha Ketoglutarate + C02“; ------ > lsocitrate . D. Acetyl-CoA + CO2 --------- > Pyruvate E. two of the above ' Which equation below best represents autotrophic CO2 fixation in the methane gens? EA. C02+H2- ------- > CH4 ' B. Sueeinyl CoA + C02; ———————— > alpha Ketoglutarate ‘ . C. Phosphoenolpyruvate + CO2 ————————— >7 Oxalacetate ' E D. Acetyl—CoA + CO2 --------- > Pyruvate E. C02 + CHs—B 12 + CoASH --------- > Acetyl-COA + B12 37. What is the key enzyme involved in oxidation of CO by the carboxydobacteria and reduction of CO2 by the methanogens? A. formate 'dehy 'drogenaSe B. fibulose bisphosphate carboxylase - ' . _ ‘D C. pyruvate decarboxylase ' D. carbon monoxide dehydrogenase E..hydrogen dehydrogenase Questions 38-40'refer to the following pathway for tryptophan biosynthesis inE. coli. Enzymes a, b, c, d and e are encoded ‘. by the structural genes of the tryptophan (17p) operon in E. coli. - .. _. . a. _a‘ ' s ._._a .. e. Statewide: emerissmta-te ‘-'-‘-~*~>- Anthrani‘ta-te ‘““""“.f“"§"- 3' "_-.-_..,» C """*‘*'- 9 """"""" > Iriliiimmm" .38. This pathway is regulated in E. coli by the process(es) of A feedback inhibition . B. end produet repression _ . ' . . . C. enzyme induction ‘ g D. catabolite repression I E. two of the above 39. This activity of which enzyme(s) is specifically inhibited by an excess of tiyptophan‘? A. a only 4 B. b only C. e only I fl} D. b> c, d, and e only . E.a,b,c,d,ande . .40 The sy nthesis of Which enzy me(s) is repressed in the presence of try ptophanfl- he- ,, - . n.-. . .. .- . .. A a only B. b only C. e only http://Www.bact.wisc.edu/Bact3 03/Eiram2 I 5/6/2005 , Exam]. ,, p ’ 'q , , , Page9 of 11 D. b, c, d, and e only 6, E.a,b,c,d,ande ‘ ' ‘ ' . . 41. An operon is A. a Series of genes regulated by a single operator B. a nucleotide region of DNA where transcription begins . C. an allosteric protein that blocks the binding of RNA polymerase to DNA . D. a region of DNA that binds an active repressor fl» E. two of the above 42. The operator region of an associated operon A. codes for the synthesis of a repressor B. is a nucleotide sequence that binds an active repressor protein C. binds RNA polymerase at the beginning of transcription of an operon _ E D. is a nucleotide sequence of an RNA molecule which is not translated into protein B. none of the above I 43. _In the pathway of lactose utilization by E. coli, when the lac operon is induced A. the lac repressor is active and transcription of the lac operon does not own B. the lac repressor is inactive and transcription of the lac operon does occur _ C. lactose cannot be transported by the cells 1’ ' g D. lactose cannot be cleaved to enter glycolysis E. none of the above " n the positive control of transcription of the lac operon (e g. catabolite repression) cy clic AMP 15 required for - .activating the lac repressor B. inactivating the lac represson I - ,, ' C. activating the CAP protein I . ‘ ' C, D. inactivating the CAP protein . E. two of the above 45. In a 1epressible operon (e. g. the tip operon) in the presence of the effector (signal) molecule the repressor protein is A. active and can bind to the operator site . B. active and cannot bind to the operator site ‘ ' . E C. inactive and cannot bind tothe operator site D. inactive and can bind to the promoter site . E. active thereby allowing RNA polymerase to transcribe the genes for that pathway Questions 46- 50 refer to the plot below which illustrates growth (as optical density) of Escherichia coli in the following minimal medium: KH2P04 2.0 g - KZI-IPO4 7.0 g A Mgso4 ' , 0.2 g (NH4)ZSO4 1.0 g http://www.bact.wisc.edu/Bact3 03/Exam2 _ . - 5/6/2005 Exam 2, glucose 1.0 g lactose 5.0 g tap water 1 liter amt density 1...; f, ”Wigwamgtmmwlmw ..: _ . . _ At), 4' a: 123 in - ‘29: 24 '23 Trméthcurslfi j 46. The pattern of growth illustrated is referred to as A. typical bacterial growth curve B. diauxic growth g 0. C. continuous culture ‘ - ' . - V D. synchronous growth E. biennial; growth 47. During what period of growth is the generation time ofthe culture the fastest? A. 0—4 hours I ' - ' B. 4-8 hours. ' C. 8-10 hours ’ ' - ' Y?) D. 10—20 hours E. 20-28 hours ' 48. At what time during the growth cycle do the bacteria utilize glucose as a source of carbon and energy? A.‘ 0-4 hours . B. 4-8 hours _ - C. 8-10 hours ' ‘ D. 10-20 hours _ ' B E. none of the above 49. At what time does the transcription of the lac operon begin? A. 0 hours B. 2 hours . ' ’ C. 8 hours ‘ , G ' D. 12 hours B. 20 hours 50. At What time does cyclic AMP begin to acoumulate in the cells? _ A. 0 hours Page 10 ofll B. 2 hours I . C http://www.bact.wisc.edu/Bact3 03/Exam2 5/6/2005 vv‘ C. _8 hours D. 12 hours B. 20 hours http://www.Bact.wisc.edu/Bact3 03/Exam2 . Page 11 of 1.1 . 5/6/2005 ...
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