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Bacteriology 303- Todar- Exam 1- Summer 03

Bacteriology 303- Todar- Exam 1- Summer 03 - -‘.L‘.bD...

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Unformatted text preview: ._,. -‘ .L‘ .bD. Archa only . 1.. s. ' «A ., ' mnfimr 193'“an -04.. .‘\_ “111:2; Bacteriology 303 Name Summer 2003 - - University of Wisconsin-Madison First Examination June 27, 2003 ©2003 Kenneth Todar University of Wisconsin-Madison Department of Bacteriology instructions; Use a No. 2 pencil. Write your name and student LD. number above on the front page of the examination. On the answer sheet print your name and identification number in the boxes indicated and blacken the corresponding circles below. ' in answering the examination questions, blacken one of the five letter circles for each of the questions. You may write on the examination but do not make stray marks on the answer sheet. Turn in both the examination 4 ‘ ' _ (question booklet) and the answer sheet at the end of the test period. ' 1. Which of the following statements regarding the universal phylogenetic tree of life is true? A. All three lines of cells ("Domains") arise from a common ancestor. v B. Archaea are the least evolved organisms - they branch off at a point closest to the root. C. Eukaryotes are the most evolved group - they have moved farthest away from the universal ancestor. D. Large cell size and acquisition of organelles in eukaryotes occurred relatively late in eukaryotic evolution - and apparently allows for an explosion of diversity. ‘ @ all of the above ‘ 2. The definitive difference between a eukaryotic cell and a procaryotic cell is based upon A. structure of chromosomes - . ribosome size nuclear structure and organization . ' ' . genomic s' e . E. nucleoti e sequences in/tfie Small subunit ribosomal RNA 3. Where do you find the organisms in Whittaker’s Kingdom ‘Protista (the "protgfts'v in Woese’s universal 0 ‘ phyloge etic tree? V . .. . A. all Donnsains WW 97- Wei—WW7“ 2 B.‘Eukarya only ' ©X\"3<- “’7 32140 W C. Bac ria only - . QV @Bacteria and Archaea 4. Viruses are not considered microorg A. are unable to reproduce ' B. lack any genetic material C. are obligate intracellular parasites D are noncellular. all of the above 5. Most of the specialized functions of eukaryotic organelles are taken over by what structure in procaryotes? A. Chloropiasts ' ' . B. chloros es outer membrane lasma membrane ’ E. nuclear membrane . ' ' 14. The overall process of nitrogen fixation in bacteria involves : ,oxidatlon of N02 _ MIXFX . V j ,-. reductiegyf NC; a H ‘ ’ . J C. oxidation of NH3 {L ‘Q V,” [L D. reduction fl N03 ° « “7/”7 N‘i’si A ND (9E. reductuowf N2 . . 15. In symbiotic nitrogen fixation between a bacterium such as Rhizobium and a leguminous plant, which member(s) of the symbiosis produces nitrogenase and RUBP carboxylase. A. The bacterium produces both nitrogenase and Rugf’fmcfiboxylase. . roduces nitrogenase an . BP carboxylase. 16. Which process(es) be aerobic respiration B. autotrophy (primary production)/\ C. methanogewesis oxygenic photosynthesis two of the above low is representative of microbial involvement in the oxygen cycle? 17. The motor apparatus of a bacterial flagellum is powered. by A. the electron transport system ' B. hydrolysis of ATP ‘ light .‘ proton motive force E. axial filaments Questions 18 22 refer to the following diagram of th muropeptide subunit f the cell wall peptidoglycan of E T coli. . . 18. The peptide bond attaching N— —acetylmuramic acid to L—alanine is shown by pointer - A. ll B. Ill C. IV @V 19 The pointer to the bond whose formation is inhibited by beta- lactam antibiotics is A. ll ' B. Ill ,, C. IV D. V 20. The arrow pointing to the bond that is cleaved by lysozyme is CED .lll .IV .V .X [710093 ‘ j ' 2‘ 1. Which component(s) of the tetrapeptide side chain is a dicarboxy—diamino acid? ¢ A. V! B. Vll @111 D. lX E. both VI and IX 22. The function of this component in the cell wall of the bacterium is to serve as a permeability barrier to all solutes\except H20 6? prevent osmotic lysis of the cell protoplast ~1- C. anchor the plasma membrane to the outer mbran'e D. regulate the passage of solutes into and out ofthe cell E none of the above 23. The interpeptide bridge of Gram-positive-bacterial murein (eg. the pentaglycine bridge in Staphylococcus aureus) connects ' ' A. Diaminopimelic acid to/D-alanine L-lysine to D-alanine . L—lysine to D-glutamate D. Diaminopimelic acid to l-lysine E. D—lysine to L—lysine Questions 24 -27 refer to the following schematic drawing of the ultrastructure of the flagellum of E. coli (after Adler). u i 24; What is the unit of measurement of the flagellar dimensions? A. angstroms nanometers C. micrometers .0. millimeters E. centimeters 2%! The flagellar motor (M) ring is indicated by pointer B .C D E 26. The flagellar support ring that is located in the periplasm in association withthe tpeptidoglycan sheet is shown by pointer 1A. B _ .E E.F 27. Which component of the flagellar apparatus did Pate find to be absent in the gliding firium, cytophaga? 28. The function of the sex (F) pilus of Escherichia coli involves A. transport of nuhfients - B. attachment of cells to inertsurfaces » C. movement of cells in a type of twit ‘ng motion . - @ransfer of DNA between bacteria'du mg conjugation . organization and folding of the bacterial chrd'nggsome within the cytoplasm 29. The capsules of procaryotic cells, including slime layers and glycocalyx, may be used to A. digest nutrients on the surfaces of cells B. attach mating cells during the process of conjugation Qattach Cells to solid surface and initiate formation of a b_iofilm“( . provide buoyancy for cells in aquatic habitats . E. regulate the passage of solutes into and out/of” the cell Questions 30-34 refers to the drawing below of the ultra structure of the cell surface of Escherichia coli. . - . '30. The structure shown by pointer lll is [email protected])mp C or omp F porin B. omp A protein C. Braun lipopoprotein D. Lipid A E. flagellar L-ring 31. The function of this structure (pointer lll above) is A. active transport of solutes across t lasma membrane B. support of the bacterial flagellum C. maintain the lateral stability of theaan’fer membrane I. anchor the outer membrane-to the underlying peptidoglycan sheet ©allow passage of solutes through the outer membrane ' 32. Bracket B defines the limits of the . A. outer membrane . lasma membrane @eriplasm . . cytoplasm E. capsule 33. Which com A. l B. ll C.V D.D <9 34. Which component ‘is peptidoglycan? A. A . ‘ B. N C. C , E.E ponent is lipopolysacCharide? 35. Gram—negative bacteria are typically less susceptible to the effects of natural penicillin thanare Gram- positive bacteria because ~ ' A. they lack a transport systemafof” penicillin , B. they degrade penicillin in the peri ic space by means of penicillinase enzymes C. they lack peptidoglycan in their wall D.__their peptidoglycan is chemical modified to Wstant to attack by penicillin ‘@-their outer membrane is an effe ctive permeability barrier 36. Polybetahydroxybutyric acid and glycogen are formed by bacterial cells as ®eserves of carbon and energy<< . reserves of nitrogen C. waste material D. molecular ligands to attac h the bacterial cells to surface receptors E. none of the above ‘ 37. This is the common inclusion observed in purple and green bacteria and lithotrophs such as Thiobacil/us ,(a colorless sulfur bacterium). ' . . sulfur B. polyph sphate . C. paraspo al c stals D. PHB granules E. magnetosgmes 0 [Z a surface structure that consists of additional protective layers ’i . no ribosomes Q c. appendages in the formof flagella and pili Y , D. relatively high rates of-respiration and energy productionv E. relatively reduced resistance to boiling and desiccation V \éa Compared to the vegetative cells that form them, bacterial endospores have 39. A chemical substance found in bacterial endospores that is not found in vegetative cells is A. diaminopimelic acid ' dipicolinic acid ' . ketodeoXyoctonoic acid D. D-glutamate E. L—lysine 40. Which of the following is not a function of the plasma membrane of proca otes? A. retention of cytoplasmic constituents as a selective permeability barrier 8. active transport of nutrients from the environment 1’ 0 @torage of reserve carbohydrates and lipids \7 D. electron transport and 02 uptake during aerobic respiration’f E. light harvesting and electron transport during photosynthesis ’( 41. The passage of molecules (solutes) across the bacterial plasma'membrane from a region of low concentration to a region of high concentration is called A. antiport (exchange diffusion) _B. facilitated diffusion C. osmosis D. group translocation ctive transport '42. Transport of glucose using a group translocation system rather than a'binding protein dependent transport system is advantageous to a bacterium because ' A. gluco , a disaccharide, is initially cleaved into its monosaccharide constituents during the transport proc - ' B. glucose can be transported infothe cell against an external concentration gradient ”T @glucose is phosphorylated as it traverses the membrane which accomplishes the first step of glycolysis and conserves ATP '~ ' D. energy is not required for transport ‘3 E. it has the effect of decreasing the internal osmola ' ywithin the cytoplasm of the cell ‘ Questions 43—45 refer to the followin coli. g diagram of the ultrastructure of the plasma membrane of Escherichia 43. The lactose transport System (lactose permease.) is an example of A. a uniport proce’ss B. an antiport prooé'ss C. a symport process 0 D an ion-driven transport system 0 of the above 44. Proton motive force of the membrane is utilized during the process of A. flagellar rotationf [L B. proline transport 10 C. ATP synthesis D. Na+ export ® all of the above 45. Suppose you could inhibit the transmembranous ATPa’se enzyme of the bacterium without inhibiting the ' membrane electron transport system. Which effect(s) on the bacterium would you expect to encounter? loss in ability to make ATP by electron transport phosphorylation B. loss in ability to establish .pmf on thmfiembrane C. loss in ability to transport sugars or amino,a€|ds using ion (proton)—driven transport systems D. loss in the ability to s l‘ ‘ ' E. two of the above. 46. In what type of bacterial culture medium listed below would yeast extract never be a component? A. an enrichment medium B. a complex medium C. a selective medium .a chemiCally-defined medium E. a solid (as opposed to liquid) medium - ‘ 47. An Archaean that is an obligate anaerobe and an extreme thermophile A. can groW°_ in the abSence of 02 B.Ican grow at 37 ° in the ppesefice of 02 C. can grow at 85 ° in the p926 of 02 @an grow at 85 ° in the absence of 02 none of the above . 48. A bacterium that is a;psychrotropfi and a facultative anaerbbe A. can grow atS ° in the absence of 02 S} _ - ' B. can grow at 5 ° in the presence of 02 C. can grow at 25 ° in the presence of 02 19-: ll of the above ‘ E. none of the above 49. A procaryote which exhibits a -' classified nutritionally as a ‘ A. extremo e B. meso e ‘ . eutropbfle a hermophile I . none of the aboVe 'O'h of 4s inimum temperature for -~'f‘“"" . 10, . :. ck of the enzymes catalase and superoxide dismutase may explain Why certain bacteria are A. iii-gable to produce oxygen radicals when exposed to 02 B. able to grow in the absence of 02 C. killed by ex D. able to respire in the mauve/OHS} E. able to respire in the W02 11 . til-.L. 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