Genetics 466- Laughon- Exam 3- Spring 02

Genetics 466- Laughon- Exam 3- Spring 02 - ‘ {ravages-r...

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Unformatted text preview: ‘ {ravages-r Hams I—umgm Genetics 46E Exam 3, 4l25lfl2 _ Name; INSTRUCTIONS Check to be sure that your exam booklet contains all 5 pages {3E- questions}. I.- Write your name on the booklet and fill in the name and ID information on the answer sheet using a #2 pencil. 1it'llhen you have completed the exam. turn in your answer sheet, exam booklet and note card {you may pick up the answer booklet and note card in 113 Genetics after the exams have been graded}. You will not receive a grade unless your name aggears on both an exam booklet and a completed answer sheet. TrUE or False [2 pt each) [about till minutes] g1 1. Southern blots are useful for determining the size of messenger RNAsm F t2. Increasing the concentration of dideoxy nucleotides in a DNA sequencing reaction is expected to increase the average length of synthesized product. i" 3. The lac repressor can act in trans when expressed from an F’ elementr [54. Promoters act in cisr while enhancers act in transnr i: E) Histone acetylation is associated with repression. F Ir 5. in humansr Eli-inactivation occurs only in cis.-r 3; k 1.; Jr; 7". Female flies heterozygous for a recessive maternal effect mutation are expected to yield “3:: q ' ii i- embryos in a 1:1 ratio of mutant vs. wild-type phenotypes, regardless of the genotype of males _‘ to which they are mated. '1’ info p8. In mosaic analysis, a “twin spot" is a clonatly-derived patch of cells that are heterozygous for the mutation in question. 1' F9. Gene targeting experiments in mice are limited to genes that are not required for viability. q"- F ii]. Proto—oncogenes are often lost by nondisjunction during tumor progression. {- Multiple choice {4 pts each} [about at] minutes] 11. Which ofthe following is the defining characteristic ofan RFLP’? {A} a high rate of changatin human populations {B} presence in transcribed regions C} a mutant phenotype polymorphism for a restriction enzyme Cleavage Elia ---- _. Genetics ass Exam a, cam ' " ' Name 2 12. Adding cAlle to a iaci' E. coii culture growing on media containing glucose as a carbon source is expected to activate the CAP protein . decrease iacZ transcription 1- inactivate the lac rephessor {D} prevent binding of lac represmr to the operator 13. Addition of tryptophan to a minimal media liquid culture of an E. coli t‘l'I-‘F? mutant would have what effect on expression of the trp operon? “'9? o_. {A} increase the rate of transcriptional initiation {W HWJLW decrease the rate of transcriptional initiation ' P @ncmase the rate of premature termination of trp operon transcription I {D} decrease the rate of premature termination of trp operon transcription '14. Which ofthe following indicates how an E colli strain witha‘iagfitlic lacZ chromosome and I an F’ harboring lacl" Ufieeziexpressp—galactesidaseiWEans expression; - means no expression} when grown with or without lactose? * L ._ pests-m” lactose without lactose lac‘kgarylm-ta + - - tilt. Ow I, age‘t > + fi masseuse _ + .l-I- [D] + + 15. BACs and YACs have which feature in common? {A} The ability to shuttle between bacteria and yeast. A yeast origin of replication he abiiity to accommodate large recombinant insertions Teiomeres 16. The restriction endonuclease Eiam H1 cleaves DNA at the sequence {stilt-KTECa In a genome that is Sti‘iti GC, what is the expected approximate average distance between BamHl sites? {assume there exists no bias against such sites} (A) zoo bp 1am a: Limb ,Dflfl bp )2o.coo bp TD. Removing the zinc fingers from a transcripfiflnfactdr wouid be lilter to disrupt its ability to contact the genEral transcriptional machinery disrupt its ability to bind EMA disrupt its ability to bind a histone acetylase * { i disrupt its pattern of expression 13. Which of the following is tme of Drosophila pair-mle genes? {A} Ate expressed matelfnally. {g Specify the number of segments. RWEM‘LWE 1| Act downstream of segment polarity genes. I @ When mutated result only in anterioriy—directed transformations. ". Genetics 455 Exam EMPE-Efflfiugr—whlacgei 3 1e. Assuming F‘CR occurs at maximal efficiency. 2i] rounds of amplification is expected to generate approximater how many doublenstranded copies of the amplified segment if the starting template is a single DNA molecule? {A} an {a)1ooo c 2oc.ooo 1f ,ooo,ooo 2o,ooo.ooo @. Mutational disruption of all repressor binding sites in the even-skipped strips 2 enhancer woul be expected to expand strips 2 in both anterior and posterior directions . xpand stripe 2 in only one direction _ contract the strips from both the anterior and posterior direction {chontract the stripe from only.r one direction 21. identify the map compatible with the following sizes of DNA fragments generated by restriction endonuclease cleavage of a EDGE! lop plasmid {the maps are linear representations of circular maps with the site at nucleotide fl shown again at nucleotide 3WD}. Kpnl = sooo hp P5u=1roo +11oo + sock- xraei = 25cc + cos a Kpnl + Pstl = 11cc + see + sac + soc-r Kpnl + Xhol = rsoo + soc + soc . Pstl + Xhol = moo + ass + 3pc + 2pc +2oo F'stl —— ————————— --I<pnl --------- --XhoI---PstI--Xhol— -------------------- --Pstl--F‘stl o soc 14cc lroo1soo 2soo soco .m ---- “"355 ---- 4o? ,____a_rag___ @) Kpnlm—m—nXhofizF‘sfl‘L—Xhol ------------- --—F’stl-‘~t—E’stl ——————————— —-—-Kpnl o._.. use... soo soo ($1 oo 1soo 21cc sooo __——--t '__..E '-r-.-._ rficp H (c) Xhol-—-F'stl--><hol ------------------- --PstI—Pstl ------------- --Kpn| -------- --Xho| o soo soo 14oo1roo ssoo scan to) Xholu-PstluXhol .......... --Kpnl ................. --Pstl---Pstl ................. --Xho| o soo soo 11cc 2ooo 22cc sooo oss of heterozygosity Gene amplification {C} Formation of chimeric fused genes {D} Capture of cellular genes by retroviruses 2 . Which of the following is n_ot a mechanism for oncogene activation“? ii Genetics 455 Exam 3. 4. .. 23. The infrequent occurrence of spontaneous retinoblestornee is clue to the low probability of inactiyating just one copy of Rb be low probability of inactiyating both copies of Rb ( the low probability of activating just one copy of Rb {D} the low probability of eotiyeting both copies of Rh Which function would be most Iiltelyto be inyolyed in tumor suppression? nhibition of the cell cycle [3 inhibition of a cell cycle checkpoint inhibition of oooptosis (D) inhibition of DNA repair 25. A methodology that is usetui for rapidly determining the expression profiles of thousands of genes: (A) Automated "shotgun" genomic DNA sequencing (B) Western blots BLAST computational searches @INA microarray technology 26. lChoose the regulatory model that best fits the following patterns of transcripts for geneX. geneY and geneZ in wild-type and mutant embryos {transcripts indicated in black}. Gone I Gene ‘r’ Gene 2 rnRNA rnRNA rnFtNA G e o © 6:: W 6g) :5 O bit #90 (A) geneX | ---- ~- geneY u—n—lgeneZ [B] geneX ---- --! gene‘r‘ I ---- --geneZ {G} geneX -----| geneY I"_"1gene2 geneX I ---- -- gene‘r" geneZ Genetics 466 Exam 3, #2502 Name] 2?. choose the pathway that best fits the following phenotypes of the hypothetical sex determination mutants sexA-sexfl. XX X‘r‘ wiid-type female male sexA' female female— sexB' ternaie female-F sexG' male male sexD' ~-'"--'—---v-——~‘W“1’hale male _ sexA'sext? ....':{ematet»- female,- 9“} seflisexfli—wflmflfmaie male ain‘t-1:} ,{nwL sexB’ sexG female-i female”??? . em gash” ' sexifi'aaxDT—--r-—“—""*-amaie male FIVE) . [A] seXG ---- —-| sexA ----- --| ssXD ---- "isexB {B} sexfi -——--| seXA ——-u-| seXC — ---- --|sexD (C) {sexC sexfl} ----—--|I[sexA sexB] sexD -----| {sexfl sexAi- ----- --| sexG cl @ sexC ------1 {semi sexfilmme—l sexD J 23. Which of the following is a property' of enhancer elements“? Respond to activators but not repressors @Function independent of orientation Are located within 5.0% base pairs ofthe regulated gene {D} Never occur within introns . . chromosome hypoactivation “tame”- “a” ‘ heterochropjatin M “a {C} expression of xisf protein {D} insulators mw-i'u-mm Genetics 466 Exam-.3,=éti‘25ifl2" ' M 3d. Below is a pedigree shewning transmission at a recessive disease trait [full shading indicates effected individuals, half-shaded indicates carriers}, along with twe Seuthem bis-ts displaying EceRI-digested DNA frem each individual {indicated ahetre each lane}. One diet is hybridized with Prehe- A. the ether with Probe B. Nut Mi Mi with”; Which cf the fciiewing is censistent with these data? * II The disease trait is linked to Fred‘s, A. - (9 The disease trait is linked it: F'rdbe El. } The disease trait is linked to bethiprdhes. {D} The disease trait is linked to neither praise.- E (Jane.ch “this ma Rmhfirhea I‘w ...
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This note was uploaded on 04/07/2009 for the course GENETICS 466 taught by Professor Staff during the Spring '08 term at Wisconsin.

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Genetics 466- Laughon- Exam 3- Spring 02 - ‘ {ravages-r...

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