Genetics 466- Exam 1- Spring 00

Genetics 466 Exam - Genetics 400 Exam 1 2t24l'00 Name INSTRUCTIONS 1 1li'lr'n'te your name on each page of this exam booklet and return it to the

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Unformatted text preview: Genetics 400 Exam 1. 2t24l'00 Name INSTRUCTIONS: 1. 1li'lr'n'te your name on each page of this exam booklet and return it to the proctor along with the scantron answer sheet when you finish. You will not receive a grade for this exam unless your name aggears on both an exam booklet and a oomgieted answer sheet. 2. Use a #2 pencil to fill in your name and student LD. number on the scantron answer sheet. 3. You may use a 3x5 inch information noteoard, but you may not write on the card. or leave the room with the card. the exam. or the answer sheet. 4. You have 2 hours to complete the exam. Truei'False [2 points each} [ about 10 minutes] 1. Microsatellite sequences are useful for genetic mapping. T 2. RNA primers are degraded by DNA polymerase I. T 3. The 5' cap stabilizes eukaryotic mRNAs- T 4. In a genome that is 50% BC. the 4 bp restriction site for Hae l|lr SEEDS—3'. is expected to occur on ayerage every 04 bp. F 5. A 5’-CAA—3‘ codon can be translated by a tRNA with a fi'—Gt..|l.l—3r antioodon. F E. Mutation of a guanine to thymine is a tmnsyersion. T 1". DNA replication occurs in a 3'~to-5' direction. F 0. The direct repeats that flank an is element have the same sequence at any site of insertion. F 0. Failure of two mutations to complement generally indicates that they do not affect the same gene. F 10.A single amino acid can be specified by multiple codons. T Multiple choice {4 points each) [about 50 minutes] 11 . A nucleic acid with a base composition of 20% adenine. 20% cytosine, 30% guanine and 30% uracil is: {A} single—stranded RNA {B} single-stranded DNA {C} double-stranded RNA {D} double-stranded DNA Genetics 466 Exam 1, 2l24r'fll] Name 12. Meselschn and Stahi demonstrated that: {A} Newly synthesized DNA strands have the same density as parental strands. {El} Newly synthesized DNA strands are more dense than parental strands. {C} Newly synthesized DNA strands are paired with parental strands. K {D} Newly synthesized DNA strands are paired with other new strands. 13. The enol form of guanine is thought to pairwith: {A} Adenine {B} Thymlne s {C} Guanine {D} Cytosine 14. Thymine dimers are not repaired by: {A} Nucleotide excision repair {B} Ease excision repair 3*: {C} Light~dependent repair {D} Post replication repair Jr: credit for either or both 15. Two separate single base-pair substitutions at a particular codon result in substitution of the amino acids glutamic acid and Ieucine. The original amino acid is: {A} Tyrosine {B} 1Glutamine {C} Histidine {D} Arginine 16. Is used to measure the size ofa specific mRNA: {A} Southern blot {B} Western blot {C} Northern blot K {D} PCR 11'. Acts after DNA polymerase III in the process of DNA replication: {A} F'rimase {B} DNA polymerase I .‘r: {C} Helicase {D} Single-stranded DNA binding protein Genetics 466 Exam 11 2l24i'flfl Name 18. Identity,r the map compatible with the following sizes of DNA fragments generated by restriction endonuclease cleavage of a sane hp plasmid: Eco RI = Shut} hp Eco RI + Barn HI = 1WD + BUG + SUD Elam HI = 25i3ll2|I + EDD Eco RI + Xha | = 15cc +1flflfl + EDD Xhal=2flflfl+ tflflfl BamHl+Xhai=13flfl+1flflh+5flfl+2flfl {A} Eco RI -—Xha I ————————— ——~————-Bam le—Bam Hl—Xha |———-—Eco RI I] Still] 13m 2300 25m U {B} Eco RI ——— —Eiam HI--Kha | — -------------- "Xha |--Bam HI --—-Eco RI El BUD 13cc ssoc 25cc [i {C} Eco RI m-——Xha | —Bam H| — ————Xha l—Bam Hl-Eco RI El 1101} 15m] 2soc 23131} D {D} Eco Rl———Xha I ——————}(ha i—Etam HI ——Bam HI —————— ——Eco RI is 0 5E":- 15flfl 1WD 220C! [l 19. If 1043 gm of a unique 300 hp PER—amplified human microsatetlite fragment is the minimal amount necessaryr for reliable detection as a single hand by gel electrophoresis. how much genomic DNA is necessary to detect heterozygosity for a 1310 his 1invariant using 2D rounds of amplification? Assume the human haploid genome is 3 x 10 hp. {A} 2 x 11343 gm {B} 11343 gm {C} 5 1: 1H? gm {DJ 2 x 1ti'T gm .5: {E} migrn 10. The splice donor sequences of pre—mRNAs {A} Are located at the 5‘ ends of exons. {B} Are located at the 5' ends of introns. .‘r'I {C} Form Iariets with the splice acceptor sequences. {D} Are recognizable within spiiced transcripts. Genetics 4% Exam 1r 2f24iiJiJ Name 21. The reannealing kinetics shown below are for sheared DNA (~5flfl hp average) from a metazoan with a haploid genome of 5 1t 1t)? hp. From these data choose the correct statement from A thm D below. The following formulas may be useful in deducing the answer: Cafmfraction = fraction 3: Gang; kinetic complexity = Cotwfraction x 1D5 ; repetition = fraction 1-: genome sitteir kinetic complexity lCfl . 8|] % single- stranded DNA fill] 49 2D {A} The largest fraction is the least complex {B} This genome contains three distinct kinetic populations {C} The smallest fraction has a kinetic complexity of it}? ‘x {D The fraction with slowest kinetics has average repetition of 2D. 22. Which of the following is not a component of a yeast shuttle vector? (A) A bacterial replication origin {B} An autonomously replicating sequence {C} A antibiotic resistance gene {D} A telomere a: 23. tRNA synthetases {A} Couple amino acids to charged tFtNAs (B) Bind specific amino acids if. {C} Contain a proofreading peptidyl trensferase {D} Process tRNA transcripts to their mature forms 24. Telomerase contains the following: {A} A ribczyme that catalyzes template-independent 3'-to-5" DNA synthesis {3} A rihczyme that catalyzes primer-independent 5'-to-3‘ DNA synthesis (C) An RNA that functions as a primer for DNA synthesis {D} An RNA that functions as a template for DNA synthesis K Genetics ABE Exam 1, 2i24iflfl Name 25. Which of the following amino acids is most Iii-rely to be substituted for Asn in mutant proteins that arise as the result of mutagenesis with the alkylating agent EMS? {A} lle {3} LEE {C} Ser X {D} Thr 26. The peptidyl transferase reaction requires {A} Elongation factor-Tu is" {B} Elase pairing between one codon and one anticodcn ({3} A charged tRNA bound at the P site [D] Release factor 2?. Nucleotide excision repair does not require {A} DNA polymerase l {B} ligase {C} glycosylase A: {D} UwD helicase 23. A cross between a Drosophila P female and an M male results in {A} A normal hybrid lacking F’ elements {B} A normal hybrid containing repressed P elements ({3} A dysgenic hybrid containing autonomous P elements {D} A dysgenic hybrid containing nonautonomous P elements 29. Which of the following is least likely to be polymorphic? {A} EST (El) micrcsallelite {C} minisatellite {D} RFLP Genetics 456 Exam 1. 2i24fflfl Name 3D. Five yeast mutants are unable to grow on minimal media without the amino acid tryptophan. These are tested for growth on minimal media supplemented with metabolic precursors to tryptophan. designated compounds A—Et with the following results: Mutant A E Compoélndtested D E E ,= C ‘i — - + ‘ ‘ 2 - + + + — 3 - _ _ _ _ 4 v + + + + 5 - + + _ — Identify the order of gene action and metabolites that can be inferred from this data: {A} 4 2 5 ‘l 3 A E D B C Trp it {a} s 1 s 2 4 C B D E A Trp (C) 4 2 5 1 3 C E'- D E A Trp {D} 3 1 5 2 4 ...
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This note was uploaded on 04/07/2009 for the course GENETICS 466 taught by Professor Staff during the Spring '08 term at Wisconsin.

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Genetics 466 Exam - Genetics 400 Exam 1 2t24l'00 Name INSTRUCTIONS 1 1li'lr'n'te your name on each page of this exam booklet and return it to the

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