This preview shows pages 1–2. Sign up to view the full content.
This preview has intentionally blurred sections. Sign up to view the full version.View Full Document
Unformatted text preview: : Experiment 17 Enzyme Kinetics of . E coli Alkaline Phosphatase Part 5 Biochemistry 332L-- 3 19 09 , The actual lab work for this experiment should be fairly brief and consists of small modifications to the activity assays you have been doing . with your enzyme all along I want you to use any extra lab time to calculate the values of K m and V max for your enzyme from the data you , obtain so we can be sure you have reasonable numbers for next week s lab . on inhibition Materials . , 0 5 mM PNPP solution freshly prepared . - . 0 2 M Tris HCl pH 8 0 Stage 4 Enzyme Before Lab . 1 , When we did the chromatography purification to create Stage IV enzyme you . did an activity assay on it Calculate how much dilution is necessary to ~ / . . achieve an activity of 60 mU 0 2 mL 2. , , - Review from class the material on the Michaelis Menten equation and how you calculate K m and V max from a set of data that includes measurements of , reaction velocity v , , [ ]. and substrate concentration S...
View Full Document