2007 Final - CHEM114C Final Name ID Location Code Please...

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CHEM114C Name ______________________________ Final -1- June 11, 2007 ID#________________________________ Location Code__________________ Please write your name on each page and look through the entire exam before beginning your answers. Please take great care in reading the question before beginning your answer. The maximum number of points for a question may indicate how extensive the expected answer is. Your exam should have 10 pages including this cover page, with a total of 9 questions. Be certain that all work you want graded is written legibly and in permanent ink. Your score 1. (25)_________ 2. (20)_________ 3. (25)_________ 4. (20)_________ 5. (25)_________ 6. (25)_________ 7. (20)_________ 8. (20)_________ 9. (20)_________ Total (200)______________ This FINAL counts 50% towards your final grade in this course.
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CHEM114C Name ______________________________ Final -2- 1. DNA damage and repair (25) 1.1. How does nitrous oxide modify cytosines and what mutation results? (5) 1.2. What type of repair is initiated by DNA glycosylases, and what is the key enzyme for this repair? (4) 1.3. What causes pyrimidine dimers to form? (2) 1.4. What repair system, found in all cells, repairs pyrimidine dimer and other DNA lesions in which bases are distorted from their normal position (2) 1.5. Name 4 enzymes involved in this repair system in E. coli . (4) 1.6. Draw a simple diagram showing the steps of this repair mechanism and when each enzyme is required. (6) 1.7. Name a human disease that is caused by a defect in this repair system. (2) 1.1. cytosine to uracil (Fig.24.28) leading G:C to A:T mutation 1.2. Base excision repair (BER), AP endonuclease 1.3. UV irradiation 1.4. Nucleotide excision repair (NER) 1.5. UvrABC endonuclease, UvrD (helicase), Pol I, DNA ligase 1.6. Figure 24.33 1.7. Xeroderma pigmentosum, or Cockayne Syndrome
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CHEM114C Name ______________________________ Final -3- 2. Eukaryotic replication (20) 2.1. Because human chromosomes are linear they require a special mechanism that maintains the ends. What are the ends called? Name the enzyme and its cofactor responsible for maintaining them (4). 2.2. Draw the model for the lagging strand synthesis of the ends, that avoids shortening of the chromosomes during replication (8) 2.3. What is the other DNA structure that maintains chromosome stability in eukaryotes and what is its function? (4) 2.4. Can you study which sequences are important for these processes in a cell-free assay system? Briefly describe the assay that can be used. (4) 2.1. telomeres, telomerase, RNA template is cofactor 2.2. Fig.24.24 2.3. centromeres; important for chromosome segragation: microtubules attach to it. 2.4 no. A complementation assay in yeast: use circular chromosome containing origin of replication, then add centromere sequence to get good segregation. When linearized, add telomere sequences so that ends are maintained.
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CHEM114C Name ______________________________ Final -4- 3. Eukaryotic gene structure and post-transcriptional processing (25)
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2007 Final - CHEM114C Final Name ID Location Code Please...

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