E11 - E1. A. Four generations: 7/8 light, 1/8 half-heavy...

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Unformatted text preview: E1. A. Four generations: 7/8 light, 1/8 half-heavy Five generations: 15/16 light, 1/16 half-heavy B. All of the DNA double helices would be 1/8 heavy. C. The CsCl gradient separates molecules according to their densities. 14 N-containing compounds have a lighter density compared to 15 N-containing compounds. The bases of DNA contain nitrogen. If the bases contain only 15 N, the DNA will be heavy; it will sediment at a higher density. If the bases contain only 14 N, the DNA will be light; it will sediment at a lower density. If the bases in one DNA strand contain 14 N and the bases in the opposite strand contain 15 N, the DNA will be half-heavy; it will sediment at an intermediate density. E2. A. You would probably still see a band of DNA, but you would only see a heavy band. B. You would probably not see a band because the DNA would not be released from the bacteria. The bacteria would sediment to the bottom of the tube....
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