{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

S18 - S1.RNA was isolated from four different cell types...

Info icon This preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
S1.RNA was isolated from four different cell types and probed with a radiolabeled cloned gene that is called gene X. The results are shown here. Explain the results of this experiment. Answer: In this Northern blot, a dark band appears in those lanes where RNA was isolated from muscle and sperm cells but not from liver and nerve cells. These results indicate that the muscle and sperm cells are transcribing gene X, but the liver and nerve cells are not. The muscle cells show a single band, while the sperm cells show this band plus a second band of lower molecular mass. An interpretation of these results is that the sperm cells can alternatively splice the RNA to produce a second RNA containing fewer exons. S2. In the Western blotting experiment shown here, proteins were extracted from red blood cells obtained from tissue samples at different stages of human development. The primary antibody recognizes the ß -globin polypeptide that is found in the hemoglobin protein.
Image of page 1

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern