S18 - S1.RNA was isolated from four different cell types...

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S1.RNA was isolated from four different cell types and probed with a radiolabeled cloned gene that is called gene X. The results are shown here. Explain the results of this experiment. Answer: In this Northern blot, a dark band appears in those lanes where RNA was isolated from muscle and sperm cells but not from liver and nerve cells. These results indicate that the muscle and sperm cells are transcribing gene X, but the liver and nerve cells are not. The muscle cells show a single band, while the sperm cells show this band plus a second band of lower molecular mass. An interpretation of these results is that the sperm cells can alternatively splice the RNA to produce a second RNA containing fewer exons. S2. In the Western blotting experiment shown here, proteins were extracted from red blood cells obtained from tissue samples at different stages of human development. The primary antibody recognizes the ß -globin polypeptide that is found in the hemoglobin protein. Explain these results.
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This note was uploaded on 04/07/2009 for the course BIO 325 taught by Professor Saxena during the Spring '08 term at University of Texas at Austin.

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S18 - S1.RNA was isolated from four different cell types...

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