lab 5 - Caroline Ho Thin Layer Chromatography of Analgesics...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Caroline Ho Thin Layer Chromatography of Analgesics Purpose: In the lab, the TLC behavior and the R f values for the components of some Over-the Counter NASID’s in four different solvent systems are determined to explore polarity concepts. This data is then used to identify an unknown pain killer. Discussion: Typically, the greater the distance and the more spots visible on the plate indicate a better solvent. In addition, it is better not to choose solvents that are in the extreme in terms of polarity. Low polarity solvent usually flows without moving the sample, causing no separation. Likewise, high polarity solvents sweep the sample right along with it, also leading to no separation. Also, mixed systems are optimal because their polarity is easily adjustable. In our experiment, 10:10: 1 toluene: ethyl acetate: acetic acid and 98: 2 ethyl acetate: acetic acid were chosen as our solvents. 60: 40 hexane: ethyl acetate was eliminated because it did not produce good results. The results were different compared
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 04/08/2009 for the course CHEM Chemistry taught by Professor Nitche during the Spring '08 term at University of California, Berkeley.

Page1 / 2

lab 5 - Caroline Ho Thin Layer Chromatography of Analgesics...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online