22 BIO 326R Transduction

22 BIO 326R Transduction - BIO 326R Transduction...

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BIO 326R Transduction
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Transduction Transfer of genetic information by a phage particle Two types: generalized and specialized Generalized May involve any host gene Specialized Involves genes located near prophage
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Generalized transduction – phage P1 P1 can be lysogenic or lytic It replicates to form a long concatemer of DNA containing several genomes of phage An endonuclease cleaves the concatemers into genome size fragments Host DNA will also be fragmented Packaging machinery cannot distinguish host DNA (bacterial genome) from phage DNA Packaging of DNA by phage P1 is based on size – it selects DNA fragments of about 90kb. Larger or smaller DNA fragments do not get packaged. Most packaging will be done with phage DNA but ~0.1% are from the bacterial genome Most, but not all, of host DNA will be wrong size to get packaged Most of processed phage DNA will be correct size Infection Phage DNA replication Endonuclease activity, production of capsids and packaging enzymes Packaging DNA into phage particles lysis
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P1 phage and generalized  transduction Phage mixture from previous slide undergoes second round of infection Most recipient cells will receive phage DNA and undergo a lytic phage P1 infection. Some will receive a copy of
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22 BIO 326R Transduction - BIO 326R Transduction...

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