26 BIO 326R Immune cells and markers

26 BIO 326R Immune cells and markers - BIO 326R Immune...

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BIO 326R Immune cells, inflammation
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A few TLAs APC – Antigen Presenting Cell (usually B cells, macrophages, monocytes, dendritic cells) CAM – Cell Adhesion Molecule (iCAM) CBC – Complete Blood Count CSF – Colony Stimulating Factor (GM-CSF, etc [or Cerebrospinal Fluid]) CTL – Cytotoxic T Lymphocyte ELISA – Enzyme Linked Immunosorbent Assay Fab – or F(ab) - Free Antigen Binding (antigen binding domain of an antibody) Fc – Freely Crystallizable (contains constant domain of antibody) HLA – Human Leukocyte Antigens (different name for MHC) iDC – immature Dendritic Cell IFN - Interferon IgH – Immunoglobulin Heavy Chain IgM, IgD, IgG, IgE, IgA – Immunoglobulin of given isotype or class IL-1 – Interleukin 1 (also 2, 3, etc) LPS - lipopolysaccharide mAb – Monoclonal Antibody (antibody preparation specific for one single epitope) MBP – Major Basic Protein (product of eosinophils) MHC – Major Histocompatibility Complex (class I and class II) aka HLA NK – Natural Killer PCR – Polymerase Chain Reaction TCR – T Cell Receptor (expressed on T cell surface) TLR – Toll-Like Receptors TNF – Tumor Necrosis Factor WBC – White Blood Cells
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Cellular markers CD – Cluster of differentiation Antibodies (and other things) were/are used to differentiate different cells. e.g. sheep erythrocytes bind to CD2. Adding sheep erythrocytes to a population of WBC will cause some of them (those that express CD2) to bind to (cluster) the sheep erythrocytes, forming a “rosette.” Adding antibodies to CD2 to this assay prevents formation of the rosette. The cells that express CD2 are said to be CD2 positive (usually written as CD2 + ) There are ~300 CD markers The CD molecules are cell surface proteins and glycoproteins Most are receptors or coreceptors Most have a known function Different types of immune cells will express different repertoires of CD markers CD4: subset of T cells, coreceptor for MHC class II CD16: Neutrophils, NK cells, Macrophages, receptor for IgG Fc Cells can often be identified or classified by determining what CD markers they display CD4+ or CD8+ is (probably) a T cell When a B cell progresses from the mature stage to the plasma cell stage, it goes from CD138- to CD138+
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Immune cells Divided into two categories, lymphoid and myeloid Lymphoid: B cells, T cells and NK cells Myeloid: all the other WBCs *Dendritic cells may be lymphoid or myeloid in origin
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Lymphoid cells Agranular cytoplasm, usually have a large nucleus, small cytoplasm Agranular means no granules, the cytoplasm looks “smooth” B cells B stands for Bursa The Bursa of Fabricius is an organ found in birds. It produces B cells and this was where B cells were first discovered. In mammals, B cells are produced in the bone marrow Produce antibodies (also called immunoglobulin)
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26 BIO 326R Immune cells and markers - BIO 326R Immune...

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