CELL BIO Lecture1_2009slides

CELL BIO Lecture1_2009slides - BIO320 2009 Lecture 1...

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Unformatted text preview: BIO320- 2009 Lecture 1 Introduction Methods in Cell Biology Alberts, 9-1 L ig h t M ic r o s c o p e Alberts, 9-2 Alberts, 9-3 Alberts, 9-6 Alberts, 9-7 Alberts, 9-11a 1 F o u r T y p e s o f L ig h t M ic r o s c o p y B r ig h t F ie ld P hase N o m a rs k i o r D IC D a r k F ie ld Alberts, 9-7 Alberts, 9-8 EM Im m u n o flu o r e s c e n c e F lu o r e s c e n c e M ic r o s c o p e : O p tic s Alberts, 9-17 Alberts, 9-13 D e te c tin g P r o te in s fo r Im m u n o flu o r e s c e n c e G F P : G r e e n F lu o r e s c e n t P r o te in Alberts, 9-18 Alberts, 9-24 2 T a g g in g P r o te in s w ith th e G r e e n F lu o r e s c e n t P r o te in DNA mRNA G e n e fo r G F P G e n e fo r P r o te in o f In te r e s t V is u a liz in g th e C y to s k e le to n w ith G F P - ta g g e d P r o te in s P r o te in GFP P r o te in o f In te r e s t V a r ia n ts o f G F P : a R a in b o w o f C o lo r s ! F lu o r e s c e n t P r o te in s & D yes F o r e v e r y flu o r e s c e n t m o le c u le th e E m is s io n w a v e is lo n g e r th a n th e E x c ita tio n w a v e Alberts, Q9-3 Alberts, 9-14 M u ltip le F lu o r e s c e n t P r o b e s c a n b e u s e d in a s in g le c e ll C o n fo c a l F lu o r e s c e n c e M ic r o s c o p y Alberts, 9-15 Alberts, 9-20 3 Conventional F luor. Microscopy C o n fo c a l F lu o r . M ic r o s c o p y O p t i c a l S e c t i o n s v i s u a l i z e d b y C o n f o c a l F l u o r. M i c r o s c o p y Alberts, 9-21 Alberts, 9-22 H o w to g e t th in g s in to c e lls L ig h t v s . E le c tr o n M ic r o s c o p e Alberts, 9-34 Alberts, 9-42 Alberts, 9-45 Alberts, 9-50 4 S D S - P o ly a c r y la m id e g e l e le c tr o p h o r e s is ( S D S - P A G E ) Alberts, 8 -19 Alberts, 8 -17 S D S - P o ly a c r y la m id e g e l e le c tr o p h o r e s is ( S D S - P A G E ) Alberts, 8 -18 Alberts, 8 -18 W e s te r n B lo ttin g Alberts, 8 -20 5 ...
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This note was uploaded on 04/12/2009 for the course CH 53170 taught by Professor Bocknack during the Spring '09 term at University of Texas.

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CELL BIO Lecture1_2009slides - BIO320 2009 Lecture 1...

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