BIO 320 Lecture1notes_2009

BIO 320 Lecture1notes_2009 - T.OHalloran&A.DeLozanne...

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BIO 320 - CELL BIOLOGY – Spring 2009 Lecture 1 Methods in Cell Biology (January 20, 2009) Reading: Alberts, 5th Ed. Chapter 8, the following sections: Isolating cells and growing them in culture Cells can be grown in culture Purifying proteins Cells can be separated into their component fractions Cell extracts provide accessible systems to study cell functions Analyzing proteins Proteins can be separated by SDS polyacrylamide-gel electrophoresis Specific proteins can be detected by blotting with antibodies Chapter 9, the following sections: Looking at cells in the light microscope The light microscope can resolve details 0.2 microns apart Living cells are seen clearly in a phase-contrast or a differential-interference-contrast  microscope Images can be enhanced and analyzed by digital techniques Intact tissues are usually fixed and sectioned before microcopy Specific molecules can be located in cells by fluorescence microscopy
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This note was uploaded on 04/12/2009 for the course BIO 50160 taught by Professor O' halloran during the Spring '09 term at University of Texas.

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BIO 320 Lecture1notes_2009 - T.OHalloran&A.DeLozanne...

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