{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

BIO 320 Lecture 11 - Voltage clamp of the neuromuscular...

Info icon This preview shows pages 1–8. Sign up to view the full content.

View Full Document Right Arrow Icon
Voltage clamp of the neuromuscular junction. Voltage electrode Current electrode Stimulate nerve
Image of page 1

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Voltage clamp pulses +65 +25 0 -15 -25 -45 -70 Stimulate nerve Currents produced by ACh at a variety of membrane voltages. Membrane clamped to these voltages. The nicotinic Ach receptor is permeable to both Na + and K + .
Image of page 2
Conductance to Na + and K + ions in the ACh receptor as a function of membrane potential. +65 +25 0 -15 -25 -45 -70 Na + ions K + ions
Image of page 3

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
The nicotinic Ach receptor is permeable to both Na + and K + . V m I E rev ACh = -15mV I Na = I K
Image of page 4
Chemical synaptic transmission: Right: Fine structure of a mammalian central terminal, containing mitochondria, synaptic vesicles and active zones and post-synaptic densities Bottom: Three phases of transmission presyn spike activates VGCC-Ca influx Ca triggers release process transmitter diffuses across cleft and activates post-synaptic receptor Synaptic transmission may or may not involve an epsp/ipsp response (cAMP, etc) Fast synaptic transmission by Glu or GABA-Rs produce the major epsps/ipsps in CNS
Image of page 5

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
What is the relationship between magnitude of the psp and E rev for a transmitter? Pipette for transmitter application Record psp amplitude
Image of page 6
What is the relationship between the magnitude of a psp and E rev ? Example: AMPA-type glutamate receptor.
Image of page 7

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 8
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern