BIO 320 Lecture 11 - Voltage clamp of the neuromuscular...

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Voltage clamp of the neuromuscular junction. Voltage electrode Current electrode Stimulate nerve
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Voltage clamp pulses +65 +25 0 -15 -25 -45 -70 Stimulate nerve Currents produced by ACh at a variety of membrane voltages. Membrane clamped to these voltages. The nicotinic Ach receptor is permeable to both Na + and K + .
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Conductance to Na + and K + ions in the ACh receptor as a function of membrane potential. +65 +25 0 -15 -25 -45 -70 Na + ions K + ions
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The nicotinic Ach receptor is permeable to both Na + and K + . V m I E rev ACh = -15mV I Na = I K
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Chemical synaptic transmission: Right: Fine structure of a mammalian central terminal, containing mitochondria, synaptic vesicles and active zones and post-synaptic densities Bottom: Three phases of transmission presyn spike activates VGCC-Ca influx Ca triggers release process transmitter diffuses across cleft and activates post-synaptic receptor Synaptic transmission may or may not involve an epsp/ipsp response (cAMP, etc) Fast synaptic transmission by Glu or GABA-Rs produce the major epsps/ipsps in CNS
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What is the relationship between magnitude of the psp and E rev for a transmitter? Pipette for transmitter application Record psp amplitude
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What is the relationship between the magnitude of a psp and E rev ? Example: AMPA-type glutamate receptor. More transmitter makes a larger amplitude
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This note was uploaded on 04/12/2009 for the course BIO 51340 taught by Professor Zakon during the Spring '09 term at University of Texas at Austin.

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BIO 320 Lecture 11 - Voltage clamp of the neuromuscular...

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