BIO311C_FU08_Chapter_17 - Transcription and processing...

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Unformatted text preview: Transcription and processing Pages 331-336 Gene expression process by which DNA directs protein synthesis, includes two stages: transcription and translation Central dogma of modern biology What we will cover Where does transcription occur in a eukaryotic as against a prokaryotic cell? Process of transcription Pre-mRNA processing in eukaryotes Exons vs. introns Transcription & Translation coupled Figure 17.3a Prokaryotic cell. In a cell lacking a nucleus, mRNA produced by transcription is immediately translated without additional processing. (a) TRANSLATION TRANSCRIPTION DNA mRNA Ribosome Polypeptide PROKARYOTES RNA made on transcription is mRNA RNA polymerase - transcription Figure 17.3b Eukaryotic cell. The nucleus provides a separate compartment for transcription. The original RNA transcript, called pre-mRNA, is processed in various ways before leaving the nucleus as mRNA. (b) TRANSCRIPTION RNA PROCESSING TRANSLATION mRNA DNA Pre-mRNA Polypeptide Ribosome Nuclear envelope Transcription and translation not coupled. Occur in separate compartments RNA polymerase II forms mRNA RNA formed on transcription is pre-mRNA. Has to be processed to mRNA Promoters DNA sequences near the beginning of genes Promoters DNA sequences near the beginning of genes that signal RNA polymerase where to begin transcription that signal RNA polymerase where to begin transcription Terminators Sequences in the RNA product that tells RNA Terminators Sequences in the RNA product that tells RNA polymerase where to stop (encoded by the DNA) polymerase where to stop (encoded by the DNA)...
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This note was uploaded on 04/12/2009 for the course BIO 311C taught by Professor Satasivian during the Fall '08 term at University of Texas at Austin.

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BIO311C_FU08_Chapter_17 - Transcription and processing...

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