Exam I SG Set 3 QA-revised

Exam I SG Set 3 QA-revised - Study Question Sets for Exam 1...

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Study Question Sets for Exam 1 Study Question Set 3 Cytochemistry, Immunocytochemistry and Enzyme Cytochemistry How does one perform immunocytochemical localization of a protein? Locate the enzymes though their catalytic activity, supply substrates and the enzyme will  generate many molecules of a localized, visible reaction product, use antibodies to detect the  specific protein by using a primary antibody directed against the localized protein, then a second  antibody is used which is a marker-coupled antibody directed against the primary antibody, the  marker on the second antibody will show the location of such protein.  What features(s) allow one to do immunocytolocalization of more than one structure  simultaneously? The use of multiple fluorescent probes of different colors, each dye is excited by a particular  wavelength of light.  On a nucleus can be use to distinguish and observe different structures such  as the microtubules with green fluorescent, DNA of condensed chromosomes in blue and  centromeres of chromosomes in red.  Three antibodies were used for such localization of the  structures.  3 What is "Indirect Immunocytochemistry"? Using immobilized unlabeled antigen for specific protein, and a primary antibody that attaches to  the immobilized antigen, and secondary antibodies for detecting it with a group of labeled  secondary antibodies that bind to it.  The secondary antibodies have a marker so that it can be  localized. Signal amplification is achieved by using multiple secondary antibodies per primary 
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This note was uploaded on 04/13/2009 for the course BIOL 3622 taught by Professor Ms during the Fall '05 term at UMSL.

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Exam I SG Set 3 QA-revised - Study Question Sets for Exam 1...

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