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Problem Set 6 Questions

Problem Set 6 Questions - CELL BIOLOGY PROBLEM SET 6 1 What...

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  CELL BIOLOGY PROBLEM SET 6 1. What are the similarities and differences between the five junctions discussed in class,  namely adherens, synapses, tight junctions, gap junctions and desmosomes? Adherers Junction o Presence Epithelial cells  endothelial cells o Distinguished from Desosomes by cadherins Important because they are calcium dependant Important because To separate cells, need to work on the cadherin Transmembrane protein Indirectly tied to F actin Cytoskeletal protein Adhesion and strength Desmosomes o Presence Epithelial and endothelial o Spot weld o E Cadherins are important as other cadherins o Examples Human Skin  In engineered skin, there are lots of desmosomes but the  key thing is if they are connected correctly Intermediate filaments o In skin, keratin filaments Basal lamina (like basement membrane) Disease states Pemphigus Vulgaris o Autoimmune disease against desmoglein 3 Pempligus Foliaceus o Autoimmune disease against desmoglein 1 Pemphagoid o Autoimmune disease against hemidesmosomes o Skin peels off in sheets Gap Junctions
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o Electrical junctions o Many Celltypes Eye source Epithelial Endothelial o What are they? 2 cells are connected by very small pores Freeze fracture and entire gap junction is visible The pore, Connexon, look like petals of flower, and will extend  between cells Proteins Pass ions and small molecules Tight Junctions o Designed to maintain a fluid barrier o Used to be called Zonula Occulens Separated fluid compartments o Presence Kidney cells Bladder Cells Intestinal Cells o Aplical region location o Testing a tight junction for efficacy Lanthanum Hydroxide Memrane impermeable stain Uses TEM Process o Add LH in cell junction and track how far it will  travel.  o Will stop at a tight junction because cannot pass  through Problem o Takes too long, TEM Sodium Fluorescien Add to aplical surface Measure amount in basal compartment using a  spectroflurometer Synapse o Neurons
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o Neuromuscular o How does it work?
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