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Unformatted text preview: CELL BIOLOGY PROBLEM SET 4 1. What are the similarities and differences between a cell strain and a cell line? What are the pros and cons of these two types of cell cultures? • Cell Strain o Usually best option o Human cells but maybe Animal (no FDA approval) Transgenic animals with gene deletion Gene for Alpha 1,3 galatosyl gene knockout No Immune rejection; possibilities for allografts • Cell Line o Will grow FOREVER; is Immortal o Not just cancer cells o Can grow great amounts 2. What is meant by "substrate" when referring to cell cultures and list some examples. Why is a microporous membrane important for the growth of some cell types? Growth Substrate requirements • Standard cell culture dish o Used to be glass now plastic o Many cells can grow • 96 well plate / 384 well plate o Used in high throughput testing, drug testing • Beads/ microcarriers o Cells grow on surface of bead. o When to use it Used in pharmaceutical trade You can put together a closed loop system. Media flows through filter system, which works like Protein A. The filter harvests bioproducts. • Roller Bottle o Slowly rolling bottle o Used for blood cells, because there is no attaching or clotting • Microporous membranes o Has pored, put them in a dish. o Become very kidney o Because something to grow on, not just flat. 3. What is the role of biological scaffolding in tissue engineering? • Biological scaffolds o Normally, scientist need to overcome the fact that cells want to grow in monolayer o Scaffold allows for 3D growth Have a 3D scaffold, and have cells which stick. At the end, have 3D Tissue 4. What engineered tissues have been made that have biomedical applications, as discussed in class? • Engineered tissues o Human Bladder Autologous; donor is same as recipient (no immune rejection) Children 4-19 years old with Spina Bifida • Causes misconnections between spine and bladder. o ELAD- Extracorporeal Liver assist device Vitagen (Was in LaJolla) Person with liver issues. Hook person up top ELAD, series of cartridges....
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This note was uploaded on 04/13/2009 for the course BIOL 311 taught by Professor Vanbuskirk during the Spring '08 term at Binghamton.
- Spring '08
- cell biology