Problem set 4 questions

Problem set 4 questions - CELL BIOLOGY PROBLEM SET 4 1....

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: CELL BIOLOGY PROBLEM SET 4 1. What are the similarities and differences between a cell strain and a cell line? What are the pros and cons of these two types of cell cultures? • Cell Strain o Usually best option o Human cells but maybe Animal (no FDA approval) Transgenic animals with gene deletion Gene for Alpha 1,3 galatosyl gene knockout No Immune rejection; possibilities for allografts • Cell Line o Will grow FOREVER; is Immortal o Not just cancer cells o Can grow great amounts 2. What is meant by "substrate" when referring to cell cultures and list some examples. Why is a microporous membrane important for the growth of some cell types? Growth Substrate requirements • Standard cell culture dish o Used to be glass now plastic o Many cells can grow • 96 well plate / 384 well plate o Used in high throughput testing, drug testing • Beads/ microcarriers o Cells grow on surface of bead. o When to use it Used in pharmaceutical trade You can put together a closed loop system. Media flows through filter system, which works like Protein A. The filter harvests bioproducts. • Roller Bottle o Slowly rolling bottle o Used for blood cells, because there is no attaching or clotting • Microporous membranes o Has pored, put them in a dish. o Become very kidney o Because something to grow on, not just flat. 3. What is the role of biological scaffolding in tissue engineering? • Biological scaffolds o Normally, scientist need to overcome the fact that cells want to grow in monolayer o Scaffold allows for 3D growth Have a 3D scaffold, and have cells which stick. At the end, have 3D Tissue 4. What engineered tissues have been made that have biomedical applications, as discussed in class? • Engineered tissues o Human Bladder Autologous; donor is same as recipient (no immune rejection) Children 4-19 years old with Spina Bifida • Causes misconnections between spine and bladder. o ELAD- Extracorporeal Liver assist device Vitagen (Was in LaJolla) Person with liver issues. Hook person up top ELAD, series of cartridges....
View Full Document

This note was uploaded on 04/13/2009 for the course BIOL 311 taught by Professor Vanbuskirk during the Spring '08 term at Binghamton.

Page1 / 7

Problem set 4 questions - CELL BIOLOGY PROBLEM SET 4 1....

This preview shows document pages 1 - 3. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online