Problem set 3 questions

Problem set 3 questions - CELL BIOLOGY PROBLEM SET 3...

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
CELL BIOLOGY PROBLEM SET 3 Answers 1. Define and/or describe the following: a. Pulse-chase - Radioactive probe is added then washed out because it is incorporated into cell, and then you ‘chase’ it with a non radioactive probe. Takes 30 minutes. After the chase, path can be traced. b. Density gradient centrifugation – Density gradient centrifugation is a method which scientists use to separate intact organelles from a cell. Each type of organelles in a cell has a specific density and based on that density you can separate a certain type of organelle from the others. c. Protein A immunoprecipitation Protein A immuno precipitation o Technique used to purify proteins and collect proteins using an antibody and Protein A. o Protein A comes from staphaureus o How do you collect cultured proteins? Put into test tube. Put in Protein A, attached to a Bead. Protein binds, spin and collect protein d. Western Blots Western Blot o Combination of Gel electrophoresis and ‘blotting’ o Try to identify a protein using an antibody probe. o Process Run gel, flip over. Transfer onto transfer paper, which is like a sponge, using a power supply. Power supply drives proteins out of gel onto paper. Superficially looks like gel. Use indirect technique to add antibody with a tag. Quantitative; you can see an actual amount. e. SELDI-TOF/MALDI-TOF SELDI-TOF o Surface enhanced Laser Desorption Ionization o Process A Protein chip with design at your discretion Add a protein, add less than 1 microliter. Because you need so little sample, you can use for non invasive analysis of tissue fluids.
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Need a Laser to point on protein and proteins ‘fly’ off of plate. The flying proteins are detected by detector.
Background image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 04/13/2009 for the course BIOL 311 taught by Professor Vanbuskirk during the Spring '08 term at Binghamton.

Page1 / 4

Problem set 3 questions - CELL BIOLOGY PROBLEM SET 3...

This preview shows document pages 1 - 3. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online