Unformatted text preview: – another divalent cation? b. You find that when you do a similar experiment with nerve cells and add a calcium ionophore to the medium there is a rapid and very large increase in intracellular calcium followed by leakage of the Fura-2 from the cell. What other fluorescent dye discussed in class might be useful to determine if the cell membrane is rupturing due to this increase in intracellular calcium versus an active pump mechanism that is activated by high calcium that, in turn, pumps the Fura-2 out of the cell? c. You find that when you add an ATP inhibitor (a drug that prevents the formation of ATP by blocking mitochondrial oxidative phosphorylation) there is also a big rise in intracellular calcium. But in this case no ionophore is present. What might you presume about the basis underlying this observation?...
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- Spring '08
- cell biology, Adenosine triphosphate, intracellular calcium