Feb 26 notes part 2

Feb 26 notes part 2 - Anatasia Gangadin Page 1 Today Finish...

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Anatasia Gangadin Page 1 10/6/2009 Today: Finish molecular biology methods Cell membranes and cell junctions Next week: Exam 3/3/09 75 multiple choice, 6-8 from the textbook Cell Membranes History o 1839 Schleinen and Schwaan Cell Theory o Implied o 1895 Overton Had living cells, but by that time, textile revolution Started working with dyes Cells stained best with lipophilic dyes He concluded that this barrier must be lipid like o 1902 Bernstein Whatever the membrane is, it has to be impermeable to ions Predicted a (Delta) Vm, a membrane potential difference o 1917 Langmuir Langmuir Trough o Has a dish, with water o Took cell membrane, and put molecules on the surface. o Made a monomolecular layer that spread well. o Took a Bar, and moved it, and measured tension o Caused phospholipids to move together o There is a transient dip in relative pressure vs bar being moved graph o Why was there a dip? Presumed there was a flip flop; went from monolayer to bilayer o There might be a monolayer or a bilayer (conclusion) o Gorter and Grendel Used Red blood cells Figure out surface area and extract all the phospholipids We can figure out if monolayer or bilayer 2 mistakes were made that canceled each other out o Wrong surface area
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Anatasia Gangadin Page 2 10/6/2009 o Not enough extraction o At the end, they came up with phospholipids bilayer (right) Problem o RBC are concave disks
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This note was uploaded on 04/13/2009 for the course BIOL 311 taught by Professor Vanbuskirk during the Spring '08 term at Binghamton.

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Feb 26 notes part 2 - Anatasia Gangadin Page 1 Today Finish...

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