feb 19 notes - Today: Finish cell culture and tissue...

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Finish cell culture and tissue engineering Start Molecular Biology Techniques Types of cell culture Cell strain Cell Line o Will grow FOREVER; is Immortal o Not just cancer cells o Origins Embryonic I.E 3T3 cells; mouse embryo fiberglass. CHO cells (not embryonic, similar) Chinese hamster ovary cells. o Genentech uses these cells as a bioproduct factory to produce monoclonal antibodies. o Herceptin Transformed cells, derived from a tumor PC3- prostate cancer HeLa Cells- cervical cancer cells Transfect cell strain with an oncogene. Expose a cell to a mutagen Untraviolet light o Not typically used, but easy to connect to, and UV is a mutagen o Very difficult to control, but still works Chemical EMS Manipulate telomeres Telomeres are the ends of DNA ‘mitotic clock’ o Get shorter each time mitosis occurs o When it gets too short, cell dies. o Advantages Grow Lots of them o Disadvantage Unless you are looking at cancer cells, these are useless; these are cancer-like cells, meaning they are mutant and not normal cells. Growth Media What the cell needs to grow in Serum supplemented media o Up to 10%, maybe 15-20% of the media is blood serum. o Perhaps not human serum, but bovine serum. o
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This note was uploaded on 04/13/2009 for the course BIOL 311 taught by Professor Vanbuskirk during the Spring '08 term at Binghamton University.

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feb 19 notes - Today: Finish cell culture and tissue...

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