Exam 1 Spring 2009

Exam 1 Spring 2009 - Exam Average is 79.5% (low B to B-)...

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Exam Average is 79.5% (low B to B-) Principles of Cell Biology Exam 1 March 3, 2009 Directions: There are 75 questions in this exam with only one correct answer for each. All answers are to be placed on the grid sheet. Make sure that you mark the version of your exam for question #76. The version is listed at the end of this exam. 1. Transmission electron microscopy does not require which of the following in any of its applications? a. Fluorochromes b. Lead and uranium c. Epoxy plastic d. Glutaraldehyde and osmium tetroxide e. Ultramicrotome 2. Which of the following techniques requires that proteins be denatured? a. DEAE ion exchange chromatography b. SDS gel electrophoresis c. CM ion exchange chromatography d. Gel filtration e. Native gel electrophoresis 3. In terms of function, which is most closely associated with the flow cytometer? a. Guava b. ELISA c. MALDI-TOF d. cDNA microarrays e. Southern blots 4. The term “in vitro” as used in class means in a. Cell culture b. A microscope c. A MALDI-TOF d. An ion exchange column e. A living animal 5. Which of the following techniques separates DNA fragments on a gel? a. Southern blots b. Western blots c. Northern blots d. SDS gels e. Gel filtration 6. Which of the following analysis systems relies on differential speeds through a gravitational field to achieve the end result? a. Dynabeads b. Flow cytometer c. Guava d. Velocity sedimentation
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DEAE ion exchange chromatography 7. As described in class, which of the following below used insulin to purify the insulin receptor? a. Gel filtration b. Ion exchange chromatography c. Affinity chromatography d. 2-D gel electrophoresis e. SDS gel electrophoresis 8. A densitometer is not used in conjunction with which of the following techniques? a. Southern blots b. Northern blots c. Western blots d. DNA gel electrophoresis e. cDNA microarrays 9. Pathologists performing forensic tissue sample tests typically fix tissue, embed it in paraffin and then stain it appropriately. Which microscope do they use in this regard? a. Phase microscope b. Nomarski optics c. Atomic force microscope d. Bright field microscope e. Polarizing light microscope 10. Which of the following techniques “amplifies” the original material? a. Southern blots b. Polymerase chain reaction c. cDNA microarrays d. MALDI-TOF e. Fast Protein Liquid Chromatography 11. Which of the following is typically not associated in any manner with cell culture? a. Cell therapy b. Tissue engineering c. Production of monoclonal antibodies in the bioprocessing industry d. In vitro toxicology e. None of the above because they all involve cell culture in some form 12. Which of the following techniques requires the use of antibodies to identify proteins? a.
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This note was uploaded on 04/13/2009 for the course BIOL 311 taught by Professor Vanbuskirk during the Spring '08 term at Binghamton.

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Exam 1 Spring 2009 - Exam Average is 79.5% (low B to B-)...

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