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Unformatted text preview: and polymerase Overhang filling or removal: make blunt ends of restriction digested DNA fragments Taq polymerase: no 3 to 5 exonuc activity DNA repair When it happens? Replication or after replication What kind of damages induced? UV damage (bulky lesion), point mutation, chromosome breaks, simple mismatch What will happen to cell? Cell cycle arrest and induce apoptosis or cance Detect, excise, and repair Werners, Bloos syndrome mutations in helicases progeria Xeroderma pigmetosa mutations in many genes involved in DNA repair cancer prone Homologous recombination: highly conserved mechanism, can be a DNA repair mechanism DNA synapse base pair forming between complementary strands of 2 DNA molecule Holliday junctio reciprocal exchange of 2 of the 4 strands present,contains 2 pairs of strands; then become isomerized (rotate) During mitosis, recombination can also occur...
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