BiochemistryMidterm ReviewPractice Test 1- Good Review at the end of the quiz if the correct answers aren’t shown then let im know.The rest of the formulas have to be memorizedBe able to calculate Molalitiy from Molarity.Formula sheetAbsorbance at 280 method relates to the pH formulas.Absorbance at 280 methodCu (mg/ml) = 1.5(A280)-0.74(A260) X DF If dilution factor is Given so if the dilution factor is 1:15 then you plug the 15into the DF value do not multiply by 1/15. Try to convert to the lowest possible common denomicator so if you are given a dilution make sure 1 is infrontExperiment 3pH problems%IonizationExperiment number 4 RCF calculationKAV=Ve-VoVe=all the stnd protein those proteins including the unknown that will enter the bedVo= those that escape from the sides and those will be used to determine the void volume.-Blue Dextran in our experiment escaped from the sides.Ve=Tube# with highest Absorbance X Volume of each fractionAprotinin: Tube 30 (was max absorb) ; absorbance= 0.405 volume of each fraction =2ml30X2=60ml elution volume can never be in the form of a decimal so make sure you round it up.Vo= Void VolumeBlue Dextran Tube #30 Peak reached;Vo= Tube with highest absorbance X volume of each frationVo= 30 X 2ml=60 ml The void volume is the same as the Ve .

Know the significance of KAVGreater than one most of the sol in sideLess than one most of them outsideEqual to one=equally distributed between inside the gel and outside the gel.Experiment number 6-Mobility CalculationCalculation of Net charge using estimation and Henderson HasselbachOne will be HH at least but know the estimation method.If your asked to calculate the charge of only one group make sure you calculate for only that group You make not always be asked to calculate the entire charge just only the value of R in that case you would use HH and calculate R.BB questions from Pre-lab and BB will be on the test2.3.08 and 2.3.12 945am start timePart BOne graph gel fil. Or pro determination For protein determination he gave us a printout in classAnd for gel filtration he gave us a ppt.