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Anatomy lab - A The effect of Increasing the Stimulus Voltage on Twitch Tension in an Isolated Frog Muscle Objective The objective of this part of

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A. The effect of Increasing the Stimulus Voltage on Twitch Tension in an Isolated Frog Muscle Objective: The objective of this part of the experiment is to understand the relationship of voltage and twitch tension. It was to see what happens to the twitch tension in an isolated frog muscle as the voltage is increased. A secondary objective was to interpret the results and to graph them, showing the major points of interest. Method: 1. Set the stimulus voltage to .1 volt and click the stimulation button. Then click the measure button after this first record is complete and then move the cursor to Record Data and click. 2. Record the Active force (gm) in the appropriate slot of the first data table. Now change the stimulus voltage to each of the subsequent vales in the first data table and repeat the steps done for the first voltage vale. 3. After each muscle twitch is complete, just click on record data (no need to click “measure” each time) adding another row to the digital table readout. 4. Place each new active force (gms) value in the appropriate slot of the same table 5. Graph your results with stimulus voltage along the x-axis and twitch tension along the y-axis. 6. Mark the following points on your graph 1. Threshold voltage – stimulation voltage which caused the first visible twitch response. 2. Maximal Voltage – stimulation voltage at which maximum twitch response first appeared 3. Threshold twitch tension – minimal twitch tension produced at threshold voltage. 4. Maximum twitch tension – peak twitch tension at maximal voltage 7. Move the cursor to the menu bar at the top of the monitor screen and click on Experiment and then choose Multiple Stimuli.
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Data: Table 1: Stimulus voltage vs. Twitch Tension Data Stimulus Voltage (v) Twitch Tension (gm) 0.2 0 0.4 0 0.6 0 0.8 .02 1.0 .15 1.5 .43 2.0 .66 2.5 .87 3.0 1.04 3.5 1.19 4.0 1.32 4.5 1.42 5.0 1.51 5.5 1.59 6.0 1.65 6.5 1.70 7.0 1.74 7.5 1.78 8.0 1.81 8.5 1.82 9.0 1.82 9.5 1.82 10.0 1.82
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Stimulus Voltage vs. Twitch Tension -0.5 0 0.5 1 1.5 2 0 2 4 6 8 10 12 Stimulus Voltage (v) Twitch Tension (gm) Graph 1: Stimulus voltage vs. Twitch Tension graph This graph shows that as the stimulus voltage increases, the twitch tension increases until a maximum threshold is reached at 8.5 volts with a maximum tension of 1.82 gm. The first three points on the graph represent the zero tension because the
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threshold has not been reached yet to stimulate a response. The first triangular point on
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This note was uploaded on 04/30/2008 for the course BI 212 taught by Professor Kaplan during the Spring '08 term at Quinnipiac.

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Anatomy lab - A The effect of Increasing the Stimulus Voltage on Twitch Tension in an Isolated Frog Muscle Objective The objective of this part of

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