Mol Biotech Ch1-3

Mol Biotech Ch1-3 - Molecular Biotechnology Reading Notes...

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Molecular Biotechnology Reading Notes Chapter 1: This chapter is just for your enjoyment. No testable material here. Chapter 2: E. coli is a gram (-) rod from human intestines and not usually in soil and water. Generation time of 22 min at 37C. Needs oxygen for optimal growth, so shake cultures are used. Saccharomyces cerevisiae is a fungus that grown as a single celled culture. It’s brewer’s yeast (“cerveza”). It is grown like E. coli , on plates or in shake culture. Yeasts are eukaryotes so they glycosylate and fold eukaryotic proteins better than E. coli , which doesn’t glycosylate at all. Question: Why would secretion be of interest to a biotechnologist? Fig. 2.2: For each, describe the function in gram (+) secretion: SRP, signal peptide, Sec complex, signal peptidase. Draw out the chronology of secretion for gram (+) bacteria for the SRP-dependent path. Note that the final protein moves freely through the cell wall. Fig. 2.3:
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This note was uploaded on 04/29/2008 for the course BIO 4333 taught by Professor E during the Spring '08 term at Baylor.

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Mol Biotech Ch1-3 - Molecular Biotechnology Reading Notes...

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