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Mol Biotech Ch7

Mol Biotech Ch7 - Molecular Biotechnology Reading Notes...

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Molecular Biotechnology Reading Notes Chapter 7 What’s a bacterial pyrogen and why is this a problem with therapeutics made from bacteria? 30% of all mammalian proteins are glycosylated in their native host. Fig. 7.2: All eukaryotes start with the same initial high mannose tree, then trim and add different sugars afterwards. Note that yeast has a high mannose mature glycosylation pattern. The N-glycosylation consensus site is at N (asp) in this background: N-X-T/S Fig. 7.1: O-linked glycosylation. For mammalian cells, define: Transfection, transformation. What does “transformation” connote in bacterial, yeast, and plant systems? YEp vectors – yeast episomal plasmids; the “2 um plasmid” of S. cerevisiae . Humans and plants don’t have plasmids, but yeast has one. Yeast selection schemes: YEp has a wt leucine synthesis gene (LEU2) and transforms a strain with a defective LEU2 gene. Select for ability to grow on leu (-) medium. A trait used instead of antibiotic resistance.
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